生酮饮食在创伤性颅脑损伤中神经保护作用研究  

Ketogenic diet plays a neuroprotective role in traumatic brain injury

作　　者：钟务招[1] 王汉东[1] 王笑亮[1] 唐勇[1] 凌海平[1] 

机构地区：[1]南方医科大学南京临床医学院(南京军区南京总医院)神经外科,南京医学硕士研究生210002

出　　处：《医学研究生学报》2012年第5期462-465,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81070974)

摘　　要：目的生酮饮食主要用于治疗癫痫,已证实它对多种神经系统疾病具有神经保护作用,但对创伤性颅脑损伤相关作用研究甚少。文中旨在探讨生酮饮食在创伤性颅脑损伤后的抗氧化神经保护作用。方法采用出生35 d的SD大鼠,建立自由落体创伤性颅脑损伤模型,分别给予生酮饮食、正常饮食1周,然后处死测脑组织干湿重比,用Western blot和RT-PCR测血红素氧合酶1(Hemeoxygenase 1,HO-1)蛋白及基因水平。结果给予生酮饮食后,脑水肿减轻,HO-1 mRNA的表达及蛋白含量均增高。结论生酮饮食对颅脑损伤患者具有抗氧化神经保护作用。Objective Ketogenic diet （KD） has been used in the clinical treatment of epilepsy and shown to have a neuro- protective effect in some nervous system diseases. However, very few studies have been reported about its effect on traumatic brain in- jury （TBI）. This study was to investigate the antioxidant neuroprotective effect of KD in TBI. Methods A TBI model was estab- lished in 35-day-old SD rats by Freeney weight-drop. The rats were fed on KD and normal diet （ND） for 1 week and then killed for the measurement of the wet/dry weight ratio of their brain tissues. The level of hemeoxygenase 1 （ HO-1 ） was determined by Western blot and RT-PCR. Results Cerebral edema was reduced and the expression of HO-1 up-regulated in the rats of the KD group as compared with those of the ND group. Conclusion KD plays an antioxidant neuroprotective role in TBI.

关 键 词：创伤性颅脑损伤 生酮饮食 血红素氧合酶1 

分 类 号：R651[医药卫生—外科学]