罗格列酮、5-ASA和VSL#3对SW480细胞TLR_4、PPARγ、NF-κB表达的影响  

Effects of rosiglitazone,5-ASA and VSL#3 on expressions of TLR_4,PPAR γ and NF-κB in SW480 Cell

作　　者：梁红亮[1] 杨曦 欧阳钦[3] 

机构地区：[1]四川省成都市第六人民医院消化内科,成都610051 [2]核工业416医院消化内科 [3]四川大学华西医院消化内科

出　　处：《西南国防医药》2012年第7期709-712,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探讨罗格列酮、5-ASA和VSL#3对SW480细胞TLR4-NF-κB信号途径关键位点蛋白表达的影响。方法 SW480细胞分为5组,4组以脂多糖(LPS)50 ng/ml刺激24 h,另一组仅加入无LPS的RMPI 1640为对照组。刺激后,3组分别以罗格列酮(10μmol/L)、5-ASA(1 mg/ml)和VSL#3(0.01 g/ml)干预2 h。收集各组上清液,用WB法检测各组TLR4、NF-κB、PPARγ蛋白的表达,用ELISA法测量IL-8生成量。结果 VSL#3明显减少TLR4表达,促进PPARγ表达,抑制NF-κB活化;罗格列酮、5-ASA明显促进PPARγ表达,阻止NF-κB活化,对TLR4基本无影响。LPS组IL-8生成量明显高于对照组;罗格列酮、5-ASA、VSL#3干预可明显降低IL-8生成量。结论 TLR4-NF-κB信号途径是重要的信号通路,PPARγ对这条信号通路有抑制作用。VSL#3通过同时降低TLR4表达和增加PPARγ的表达,抑制NF-κB的活性,抑制这条信号通路,最终缓解炎症。罗格列酮的作用可能主要是通过增加PPARγ表达和促进其活性实现。PPARγ是5-ASA的作用靶点之一。Objective To discuss the effects of rosiglitazone,5 - ASA, and VSL#3 on the expression of proteins at the key sites on the signal pathway of TLR4- NF - KB. Methods SW480 cells were divided into 5 groups, among which 4 groups were stimulated with LPS（50 ng/ml） for 24 h and the other group was treated with RMPI 1640 which had no LPS and was chosen as the control group. After stimulation,3 groups were interfered for 2 h by rosiglitazone （10 μmol/L）,5 - ASA（ 1 mg/ml）, and VSL#3 （0.01 g/ml）, respectively. The supernatants of each group were collected in order to detect the expression of TLR4, NF - KB and PPAR y by western blotting（WB）. And formation amount of IL - 8 was measured by ELISA. Results VSL#3 significantly decreased the expression of TLR4, enhanced the expression of PPAR - y, and inhibited the activation of NF - KB. Rosiglitazone and 5 - ASA obviously enhanced the expression of PPAR - y, inhibited the activation of NF - KB, and had no effects on TLR4. In LIPS groups, the formation amount of IL - 8 was significantly higher than that in the control group. The intervention with rosiglitazone, 5 - ASA, and VSL#3 could significantly decrease the formation amount of IL - 8. Conclusion The signal pathway of TLR4- NF - KB is an important signal pathway, and PPAR has an effect of inhibition on it. VSL#3 can inhibit this signal pathway and finally relieve inflammation by reducing the expression of TLR4, promoting the expression of PPAR - y, and inhibiting the activation of NF - KB. The effect of rosiglitazone may be realized mainly through increasing the expression and promoting the activation of PPAR y. PPAR y is one of the action targets of 5 - ASA.

关 键 词：罗格列酮 VSL#3 TLR4 PPARΓ SW480细胞 

分 类 号：R392[医药卫生—免疫学]