电化学发光免疫分析检测乙肝标志物的临床价值  

Clinical value of electrochemiluminescence immune assay in detectioin of hepatitis B markers

作　　者：游媛[1] 陈岚[1] 肖鄂[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院西区检验科,武汉430056

出　　处：《西南国防医药》2012年第7期767-769,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的探讨电化学发光免疫分析(ECLIA)定量检测乙肝标志物的临床意义。方法采用ECLIA法定量检测361例乙肝患者的HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc水平,分析其与HBV-DNA水平、肝功能检测结果、临床分型的相关性。结果 HBsAg、HBeAg与HBV-DNA定量呈正相关(r=0.6336,0.4032,P<0.01),HBsAb、HBeAb、HBcAb与HBV-DNA定量呈负相关(r=-0.2582,-0.3864、-0.3568,P<0.01);乙肝标志物定量检测与患者肝功能指标均无相关性(P>0.05);HBsAg、HBeAg、抗-HBc含量在急性乙肝和亚急性重症乙肝患者中较慢性乙肝、慢性重型乙肝和肝硬化患者低(P<0.05)。结论 ECLIA定量检测乙肝病毒血清学标志物含量可反映乙肝病毒的复制水平,定量检测结果与肝功能异常无明显关联,但与临床分型相关。Objective To discuss the clinical value of electrochemiluminescence immune assay （ECLIA） in the detection of hepatitis B markers. Methods ECLIA was used to quantitatively detect the levels of HBsAg, anti - HBs, HBeAg, anti - HBe, and anti - HBe in 361 patients with hepatitis B. Analysis was made in the correlation between those indexes and the level of HBV - DNA, examination results of liver function,ad clinical classification. Results There was a positive correlation between the levels of HBsAg and HBeAg and the quantitation of HBV - DNA（ r = O. 6336,0. 4032 ,P 〈 O. O1 ）. There was a negative correlation between the levels of HBsAb,HBeAb,and HBeAb and the quantitation of HBV - DNA （ r = - O. 2582, - 0. 3864, - O. 3568, P 〈 0. O1 ）. There was no correlation between the levels of hepatitis B markers and the indexes of liver function（ P 〉 0.05 ）. The levels of HBsAg, HBeAg, and anti - HBc in patients with acute hepatitis B and sub - acute gravis hepatitis B were lower than those in patients with chronic hepatitis B, chronic gravis hepatitis B, and cirrhosis （ P 〈 O. 05 ）. Conclusion The quantitative detection of serologic marker levels in hepatitis B virus by ECLIA can show the replication ability of this virus. The quantitative detection results have no significant correlation with liver dysfunction, but are correlated with the clinical classification.

关 键 词：电化学发光免疫分析 乙肝标记物 检测 临床价值 

分 类 号：R446.11[医药卫生—诊断学]