丙泊酚预先给药对大鼠肾缺血再灌注损伤的影响  

Effect of Propofolon on Renal Ischemia-Reperfusion Injury in Rats

作　　者：姜琳[1] 刘庆宏[2] 孙灿林[1] 肖蔚[3] 戴桂红[3] 于鸿[3] 

机构地区：[1]江苏省泰州市人民医院麻醉科,225300 [2]江苏省泰州市人民医院普外科,225300 [3]江苏省泰州市人民医院病理科,225300

出　　处：《中华全科医学》2012年第8期1165-1166,1178,共3页Chinese Journal of General Practice

基　　金：南通大学自然科学基金资助项目(10Z088)

摘　　要：目的探讨丙泊酚预先给药对大鼠肾缺血再灌注损伤(IR)的影响。方法健康成年雄性大鼠36只,体重220～250 g,随机分为3组(n=12):假手术组(S组);缺血再灌注组(IR组)和丙泊酚预处理组(P组)。IR组和P组采用夹闭双侧肾动、静脉45 min后恢复灌注的方法制备大鼠肾缺血再灌注模型,S组不夹闭双侧肾动、静脉。P组于夹闭前15 min经尾静脉注射丙泊酚2.5 mg/kg,S组和IR组分别尾静脉注射等量溶剂。于再灌注后2 h留取血和肾组织标本同时处死大鼠,检测血清尿素氮(BUN)、肌酐(Cr)浓度,观察肾组织的病理学及细胞间粘附分子-1(ICAM-1)、肿瘤坏死因子-α(TNF-α)表达改变。结果与S组比较,IR组血清BUN及Cr浓度均显著升高(P<0.01),肾组织ICAM-1及TNF-α表达显著增加(P<0.01);与IR组比较,P组血清BUN及Cr浓度均显著降低(P<0.01),肾组织ICAM-1及TNF-α表达显著减少(P<0.01)。结论丙泊酚可减轻肾缺血再灌注损伤,可能与其抑制肾组织ICAM-1及TNF-α表达有关。Objective To investigate the effect of propofolon on renal ischemia-reperfusion injury（IR） in rats and the possible mechanism.Methods Thirty-six male SD rats weighing 220-250 g were randomly divided into 3 groups with 12 animals in each group： sham operation group（group S）;I/R group and IR＋ propofolon group（group P）.The renal ischemia was induced by occlusion of bilateral renal arteries and veins for 45 min followed by reperfusion.In group P,propofolon 2.5 mg/kg was injected via the tail vein 15 min before ischemia and,while the equal volume of solvent was given in group I/R.Arterial blood samples were taken at 2 h reperfusion for determination of concentrations of serum urea nitrogen（BUN） and creatinine（Cr）.The rats were then sacrificed and the kidney was removed to detect renal histopathology lesions,and the contents of intercellular adhesion molecule（ICAM-1） and tumor necrosis factor-alpha（TNF-α） in kidney tissue were measured.Results The concentrations of serum BUN and Cr were significantly increased in group I/R compared with group S（P0.01）,while the expressions of ICAM-1 and TNF-α in kidney tissue were significantly increased.The concentrations of serum BUN and Cr were significantly decreased in group P compared with group IR（P0.01）,while the expressions of ICAM-1 and TNF-α in kidney tissue were significantly decreased in group P compared with group IR（P0.01）.Conclusion Propofolon could attenuate renal IR injury and the underlying mechanism may be related to the inhibition of ICAM-1 and TNF-α expressions.

关 键 词：丙泊酚 肾 再灌注损伤 细胞间粘附分子-1 肿瘤坏死因子-Α 

分 类 号：R614.2[医药卫生—麻醉学] R364.12[医药卫生—外科学]