向日葵黑茎病菌的快速分子检测  

Fast molecular detection of the pathogen of sunflower black stem

作　　者：宋娜[1] 陈卫民[2] 杨家荣[1] 胡小平[1] 

机构地区：[1]西北农林科技大学植保学院与作物逆境生物学国家重点实验室,陕西杨凌712100 [2]新疆伊犁职业技术学院,新疆伊宁835000

出　　处：《菌物学报》2012年第4期630-638,共9页Mycosystema

基　　金：中央高校基本科研业务费专项(No.QN2009034);高等学校学科创新引智计划(No.B07049)

摘　　要：从新疆采集的向日葵黑茎病罹病植株的茎秆、叶片和花盘共分离获得20个真菌分离物。经致病性测定,证明分离物XJ011和XJ111是引起该病害的病原物。采用ITS通用引物ITS1/ITS4对XJ011和XJ111菌株的rDNA-ITS区进行PCR扩增和测序,并结合形态学特征,将该菌鉴定为麦氏茎点霉Phoma macdonaldii。同时,在rDNA-ITS的多态性丰富区域设计了一对特异性引物320FOR/320REV,建立了P.macdonaldii病菌的快速分子检测体系,能特异性检测出向日葵黑茎病菌,灵敏度可达到1fg。The diseased stems, leaves and faceplates of sunflower with black stem symptom were sampled in Xinjiang Autonomous Region, China. Twenty fungal isolates were finally isolated from the materials. Isolates XJ011 and XJ111 were found to be pathogenic to sunflower plants. ITS sequence analysis indicated the isolates XJ011 and XJ111 were Phoma macdonaldii. A pair of specific primers 320FOR/320REV were designed for rDNA-ITS of P. macdonaldii according to polymorphism region, and it can be used to detect P. macdonaldii specifically with highly sensitivity of 1 fg concentration.

关 键 词：致病性 特异性引物 rDNA-ITS区 向日葵 

分 类 号：S435.655[农业科学—农业昆虫与害虫防治]