Ghrelin免疫阳性细胞在山羊胃肠道的发育性变化  

Developmental Changes of Ghrelin-immunopositive Cells in the Gastrointestinal Tract of Goat

作　　者：张德敏[1,2] 王树迎[1,2] 尹玉涛[1,2] 刘宵[1,2] 侯衍猛[1,2] 黄丽波[1,2] 张春兰 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东农业大学动物科技学院动物生物技术与疾病防治重点实验室,泰安271018 [3]山东省牧工商总公司,济南250022

出　　处：《畜牧兽医学报》2012年第7期1042-1048,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30871832);山东省自然科学基金(ZR2009DM027)

摘　　要：为探究Ghrelin免疫阳性细胞山羊胃肠道的定位分布、形态特征与发育性变化,选用0、30、60、90、120、150和180日龄雌性济宁青山羊,按试验要求取材后进行免疫组织化学方法染色。结果显示,Ghrelin免疫阳性细胞主要分布在黏膜上皮、腺体和固有层,有"闭合型"和"开放型"2种类型。2种类型细胞在皱胃、十二指肠、空肠、回肠、盲肠和结肠均有分布,但未在瘤胃、网胃、瓣胃和直肠中检出。Ghrelin阳性细胞在皱胃中最多,由皱胃至结肠逐渐减少,"闭合型"细胞所占比例也由皱胃至结肠逐步减少。从0～120日龄,皱胃和空肠中阳性细胞数随着年龄增长而增加,150和180日龄有所下降;十二指肠中阳性细胞在90日龄达到高峰。回肠、盲肠和结肠中阳性细胞数较少,均呈现随日龄增长而缓慢增长的趋势。Ghrelin免疫阳性细胞的数量变化表明Ghrelin可能与山羊胃肠道发育密切相关。In the present study,the distribution,morphological characteristics and developmental changes of Ghrelin-immunopositive（Ghrelin-ip） cells in the gastrointestinal tract of goat were investigated.Using the method of immunohistochemistry,Ghrelin-ip cells were showed in the gastrointestinal tract of Jining Gray goat at the ages of day 0,30,60,90,120,150 and 180.Ghrelin-ip cells classified into open-and closed-type cells were found to be localized in the mucosa epithelium,glands and lamina propria of abomasums,duodenum,jejunum,ileum,cecum and colon,but not in rumen,reticulum,omasum and rectum.The greatest number of Ghrelin-ip cells was found in the abomasums and the Ghrelin-ip cell density gradually decreased from the abomasums to the colon.The percentage of closed-type cells to all immunopositive cells also decreased from the abomasums to the colon.With the goat growth,the densities of Ghrelin-ip cells in the abomasums and jejunum increased from ages of day 0 to 120,but slightly decreased at ages of day 150 and 180.There was also a steady increase in the number of Ghrelin-ip cells from postnatal day 0 to 90 in duodenum,which reached a peak and remained steady.Very few Ghrelin-ip cells were found in the ileum,cecum and colon,and the densities increased with the age growth.The change of Ghrelin-immunopositive cells in the gastrointestinal tract of growing goats suggests that Ghrelin may be involved in gastrointestinal tract development.

关 键 词：GHRELIN 济宁青山羊 胃肠道 免疫组化 

分 类 号：S827[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]