狂犬病基因工程灭活疫苗(Hep-Flury-dG株)的免疫效力  

Efficacy of protection effect induced by inactivated rabies vaccine(Hep-Flury-dG)

作　　者：施赫赫[1] 宋伟科 黄永亮[1] 刘祥茵[1] 代元元[1] 傅秋玲[1] 张莹[1] 林颖仪[1] 阳佑天[1] 徐晓娟[1] 王怡飞[1] 郭霄峰[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]增城区荔城兽医站,广州511300

出　　处：《中国人兽共患病学报》2012年第7期664-668,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(30871876);农业公益性行业专项(201103032);广东省省部产学研重点(2010A090200083)~~

摘　　要：目的检测狂犬病基因工程灭活疫苗(Hep-Flury-dG株)注射犬只后的免疫效力。方法在广州市增城区某村进行狂犬病基因工程灭活疫苗(Hep-Flury-dG株)环境释放试验,登记该村所有家养犬的信息并存档,同时进行疫苗免疫。随机采集免疫前血清样品66份,免疫后21d仍获得血清样品66份,使用ELISA方法检测样品的抗体水平。结果免疫前该村犬只抗体阳转率为26%,免疫后21d抗体阳转率达到83%。经统计分析表明免疫前后抗体水平差异具有统计学意义;性别不是影响抗体水平变化的因素,但年龄可影响抗体水平变化,成犬抗体水平的增加明显高于幼犬。结论狂犬病基因工程灭活疫苗(Hep-Flury-dG株)免疫犬只后可提供足够的保护力,有效保护犬只免受狂犬病病毒感染,因而预防狂犬病从犬-犬或犬-人的传播;为农村地区家养犬建立免疫档案,监控犬只抗体水平,使犬群间形成狂犬病抗体阳性率达到70%以上的坚强免疫带,是预防农村地区狂犬病发生的一种可行、有效的方法。In order to determine the efficacy of inactivated rabies vaccine （Hep Flury-dG） strain, we carried out an expending experiment in a village of Guangzhou. The information of all the dogs vaccinated in this research was collected. On day 0 before immunization and day 21 post immunization, the blood samples of 66 dogs were collected. The serum were separated and the antibody against rabies virus were detected by ELISA. Results showed that seroconversion were 26% before using the inactivated vaccine, while was 83% after immunization. Statistical analysis indicated that there was statistical significance be tween antibody levels before and after immunization. The gender of the dog was not a factor that could affect the antibody level, while the age was the key factor. Thus, the inactivated rabies vaccine could offer protection against rabies virus from dog＇s immune system.

关 键 词：狂犬病 基因工程疫苗 抗体 免疫效力 

分 类 号：R373.9[医药卫生—病原生物学]