广西家畜布鲁氏菌病的监测分析  

Surveillance analysis of livestock brucellosis in Guangxi Province,China

作　　者：李军[1,2] 陈泽祥[1,2] 杨威[1,2] 谢永平[1,2] 李常挺 傅启宽[4] 骆永泉 李常春 许力干[1,2] 赵武[1,2] 

机构地区：[1]广西兽医研究所,南宁530001 [2]广西畜禽疫苗新技术重点实验室,南宁530001 [3]广西隆安县动物疫病预防控制中心,隆安532700 [4]广西防城港市动物疫病预防控制中心,防城港538000 [5]广西贺州市动物疫病预防控制中心,贺州542800 [6]广西靖西县动物疫病预防控制中心,靖西533800

出　　处：《中国人兽共患病学报》2012年第7期754-758,共5页Chinese Journal of Zoonoses

基　　金：农业部公益性行业(农业)科研专项(201103008);广西科技成果转化资金项目(10100017-8);广西科技攻关项目(10100014-4);广西水产畜牧兽医局资助项目(桂渔牧财[2011]52号)资助~~

摘　　要：目的对广西家畜布鲁氏菌病进行监测。方法采用虎红平板凝集和试管凝集试验对2009-2011年广西14个市的16 143份家畜血清进行布鲁氏菌抗体监测;同时对1 070份家畜脾、胎衣、流产胎儿等样本以及10份布鲁氏菌试管凝集试验抗体阳性家畜的子宫或睾丸进行布鲁氏菌分离和鉴定。结果 2009年有1头种公猪,2010年有1头牛和8头山羊被检出为布鲁氏菌抗体阳性;经生化特性和PCR鉴定有1株从布鲁氏菌试管凝集试验抗体阳性羊内脏分离到的细菌被鉴定为羊种布鲁氏菌。对分离的羊种布鲁氏菌毒力基因VirB8的克隆测序结果显示,VirB8基因在布鲁氏菌种型间高度保守。结论虽然广西家畜布鲁氏菌防治达到稳定控制标准,但需加强家畜引种和动物流通检疫工作,防止因引种和动物流通而将布鲁氏菌引入广西。To survey the epidemiology of livestock brucellosis in Guangxi Province, China, 16 143 livestock serum sam- ples collected from 14 cities during 2009 to 2011 were detected by rose bengal plate agglutination test and tube agglutination tests. Meanwhile, Brucella isolation and identification were performed from 1 070 livestock spleen, afterbirth and aborted fetus samples, and 10 viscera samples with positive serum. The serology test indicated that 1 boar serum sample in 2009 as well as 1 cattle serum sample and 8 goat serum samples in 2010 were positive, respectively. Comparison of nueleotide sequences of VirB8 gene of 13. melitensis isolate with others Brucella showed that VirB8 gene was conservation among Brucella. Our results indica- ted that livestock quarantine inspection needs to be enhanced for prevention of Brucella ＇s intrusion to Guangxi by livestock in troduction.

关 键 词：布鲁氏菌 广西 监测 VirB8 

分 类 号：R516.7[医药卫生—内科学]