孕前低水平铅暴露对母鼠孕期及哺乳期骨转化的影响  

The effects of low pre-pregnant lead exposure level on maternal bone turnover during gestation and lactationin mice

作　　者：宋波[1] 武柏林[2] 孙素菊[1] 张荣[1] 谭凤珠[1] 牛玉杰[1] 

机构地区：[1]河北医科大学劳动卫生与环境卫生教研室,石家庄050017 [2]河北医科大学附属第二医院,石家庄050017

出　　处：《中华劳动卫生职业病杂志》2012年第7期493-496,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：河北省计划生育委员会研究基金资助项目（2011-A08）：河北省卫生厅科研基金项目（20100037）

摘　　要：目的研究孕前期低水平铅暴露对母体孕期和哺乳期骨骼铅动员的影响。方法72只雌性昆明小鼠随机分为铅暴露组、对照组，每组36只。铅暴露组小鼠通过饮水暴露于含铅量为50mg/L（以铅计）的醋酸铅，对照组小鼠饮用去离子水，4周后与健康雄性小鼠同笼并证实怀孕，分别在孕期的第1、7和14天，哺乳期的第1、10和21天取全血、股骨，用石墨炉原子吸收法、火焰原子吸收法测定铅、钙含量。结果在哺乳期末，与自身孕前相比，铅暴露组母鼠的血钙、骨钙水平分别下降了18．50％和17．75％，差异均有统计学意义（P〈0．05），血铅水平上升了65．22％，骨铅水平下降了28．45％，差异均有统计学意义（P〈0．05）。孕期及哺乳期母鼠血铅水平与骨铅水平呈负相关（r=-0．904，P〈0．01）。铅暴露组母鼠的体内的铅、钙含量，在孕期及哺乳期的各个观察阶段均与对照组的差异均有统计学意义（P〈0．05）。结论母体孕期和哺乳期发生了骨骼铅动员现象，孕前期低水平铅暴露加剧了母体孕期和哺乳期骨骼铅动员。Objective To study the effects of low pre-pregnant lead exposure level on the mobilization of lead and calcium in maternal skeleton during gestation and lactation in mice. Methods Seventy Kunming female mice were randomly divided into the lead exposure or control groups, 36 mice were exposed to lead by drinking water （50 mg/L） and 36 mice were exposed to deionized water for 4 weeks. The levels of calcium and lead in blood and femurs were measured on the 1st, 7th and 14th days during gestation and on the lst,10th and 21st days during lactation with atomic absorption spectrophotometry using a heated graphite atomizer or flame atomic absorption spectrophotometry. Results As compared with the pre-pregnant, at the end of lactation in exposure group the levels of calcium in blood and bones significantly decreased 18.5% and 17.75%, respectively, the levels of lead in blood significantly increased 65.22% and the levels of lead in bones significantly decreased 28.45% （P〈0.05）. There was a significant negative correlation between the blood lead level and the bone lead level during gestation and lactation in exposure group （r=-0.904, P〈0.01）. There were significant differences of lead and calcium levels during the gestation and lactation between exposure group and control group （P〈0.05）. Conclusion The lead mobilization in maternal bone occurred during gestation and lactation in mice, which could be accelerated by the low pre-pregnant lead exposure.

关 键 词：铅 钙 骨 孕期 哺乳期 

分 类 号：R714.2[医药卫生—妇产科学]