水中汞离子（Ⅱ）的纳米生物传感器检测法  

Detection of mercury （ H ） ions （Hg2＋） in water by a nanobiosensor

作　　者：吴文鹤[1] 洪小兰[1] 项序武[1] 姚小艳[1] 泮婷婷[1] 熊隽[1] 吕建新[1] 

机构地区：[1]温州医学院检验医学教育部重点实验室,浙江省医学遗传学重点实验室,325035

出　　处：《中华劳动卫生职业病杂志》2012年第7期536-540,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金项目（81101148）;浙江省科技计划面上科研项目（2009C33036）;浙江省自然科学基金项目（Y2081072）;温州市科技计划重大项目（Y20080080）;温州医学院2011年学生科研立项课题（wyx201101033）;温州市科技计划项目（Y20110021）

摘　　要：目的运用汞离子特异性寡核苷酸探针和纳米金，构建一种能快速比色检测水中Hg2＋的纳米生物传感器。方法对已有的汞离子特异性核苷酸探针进行优化设计，并吸附到纳米金颗粒表面，实现生物传感器的组装。利用汞离子特异性稳定T．T错配和纳米金颗粒在高盐条件下的颜色变化，从而实现对H矿的定量分析。结果制备的纳米金粒径为15nm，浓度为2．97nmol／L；成功获得2条截短的汞离子特异性核苷酸探针（9bp）；构建的汞离子纳米生物传感器，最佳盐浓度为0．5mol／L，检测Hg2＋仅需数分钟，检出限为10μmol／L，特异性为100％。结论本研究构建的纳米生物传感器，不需要标记探针或者修饰纳米金，在不使用任何特殊条件下就可以实现对汞离子可视化便捷分析，具有操作简便、检测时间短、灵敏度较高、特异性强等优点，易于推广使用。Objective To develop a nanobiosensor for rapid colorimetric detecting Mercury （II） Ions （Hg2＋） in water by mercury-specific oligonucleotides （MSOs） probe and gold nanoparticles. Methods The nanoaptasensor was assembled by adsorbing the optimized MSOs on the surface of gold nanoparticles. A direct colorimetric probe of Hg2＋ which relied on the T-T mismatches in DNA duplexes was used to selectively and strongly capture Hg2＋. Hg2＋ induces the aggregation of gold nanoparticles with appropriate amount of salts, resulting the colorchange （red to blue）. Results The diameter and concentration of the gold nanoparticle preparation were 15 nm and 2.97 nmol/L, respectively. Truncated MSOs （9 bp） showed the similar Hg2%inding activity. The optimum concentration of the NaNO3 solution was 0.5 mol/L. The nanobiosensor could detect Hg2＋ in a range of 10 -1000 μmol/L within few minutes and the specificity was 100%. Conclusion A new nanobiosensor is developed successfully for rapid colorimetric detecting Hg2＋ in water, avoiding either MSOs labeling or gold nanoparticles modification. This technique is simple, convenient and rapid detecting method with high sensitivity and specificity.

关 键 词：汞 特异性寡核苷酸探针 纳米生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]