猪整合素β_1基因CRS-PCR多态性与产仔数的关联性分析  

Analysis of association between CRS-PCR polymorphisms of integrin β_1 gene and litter size in pigs

作　　者：郭良勇[1] 傅金銮[1] 王爱国[1] 

机构地区：[1]中国农业大学动物科学技术学院,畜禽育种国家工程实验室,农业部畜禽遗传育种重点开放实验室,北京100193

出　　处：《遗传》2012年第7期879-886,共8页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:31172176);国家现代农业产业技术体系项目(编号:CARS-36)资助

摘　　要：文章采用CRS-RFLP技术对长白猪、大白猪和杜洛克猪3个品种的整合素β1基因第5外显子T32207C位点及第7外显子A35230G位点进行单核苷酸多态性分析,并将基因多态性与猪的产仔数进行关联分析。结果表明:32207多态位点的基因型效应对3个品种的总产仔数(TNB)和产活仔数(NBA)影响均不显著;35230多态位点的基因型效应对大白猪和长白猪头胎、二胎及所有胎次的TNB和NBA的影响达到显著(P<0.05)或者极显著水平(P<0.01),基因型GG、AG与AA对产仔数的影响存在差异,其效应为GG,AG>AA。可见整合素β1基因35230位点的G等位基因对大白猪和长白猪的产仔数性状有显著影响。Single nucleotide polymorphisms of exon 5 T32207C and exon 7 A35230G of integrin β1 gene were detected in Landrace, Large White and Duroc by CRS-RFLP. Association between the polymorphism and litter size was analyzed by the method of least square means. At 32207 polymorphic loci, there was no significant difference on TNB and NBA be- tween the genotypes in Landrace, Large White, and Duroc. At 35230 polymorphic loci, there was significant difference （P〈0.05） or greatly significant difference （P〈0.01） on TNB and NBA between genotypes in the first, second, and all parities in Large White and Landrace. The effects of GG and AG genotypes were different from that ofAA genotypes with the order of GG, AG〉AA. These results suggested that the effect of G allele of integrin β1 gene on litter size is significant in Large White and Landrace.

关 键 词：CRS-PCR 整合素β1基因 多态性 产仔数 猪 

分 类 号：S828[农业科学—畜牧学]