二苯乙烯苷对H_2O_2诱导血管内皮细胞黏附分子表达的影响  

Effect of TSG on expression of adhesion molecule induced by H_2O_2 on vascular endothelial cell

作　　者：张彩平[1] 杨滢[1] 田英[1] 乔新惠[1] 龙石银[1] 陈志军[1] 田汝芳[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南衡阳421001

出　　处：《中国药理学通报》2012年第8期1088-1092,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30800474);湖南省科技厅项目(No 2010SK3037);湖南省教育厅重点项目(No11A104);湖南省研究生创新项目(No CX2010B385;CX2011B378)

摘　　要：目的研究二苯乙烯苷(TSG)对过氧化氢(H2O2)诱导损伤的人脐静脉内皮细胞P-selectin、E-selectin、ICAM-1、VCAM-1和MCP-1表达的影响。方法体外培养人脐静脉内皮细胞,实验分为空白对照组、H2O2组、辛伐他汀组、TSG组,运用逆转录聚合酶链式反应和酶联免疫吸附试验分别检测P-selectin、E-selectin、ICAM-1、VCAM-1和MCP-1 mRNA与蛋白的表达。结果 200μmol·L-1的H2O2作用内皮细胞24 h后,P-selectin、E-selectin、ICAM-1、VCAM-1和MCP-1的mRNA和蛋白表达水平均明显上调;经TSG预处理内皮细胞4 h后,再加200μmol·L-1的H2O2作用内皮细胞24 h,结果显示:TSG能抑制H2O2诱导的内皮细胞P-selnecti、E-se-lectin、ICAM-1、VCAM-1和MCP-1的mRNA和蛋白的表达。结论 TSG可抑制H2O2诱导的人脐静脉内皮细胞黏附分子的表达。Aim To study the effect of 2,3,5,4＇-tetrahydroxystilbene-2-O-β-D-glucoside on expression of adhesion molecules P-selectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 induced by H2O2 on human um- bilical vein endothelial cells （HUVECs）. Methods HUVECs were cultured in vitro. The experiment was divided into four groups ： control group, H2O2 group, positive group, and TSG group. The expression of P- selectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 mRNA and protein were respectively detected by reverse transcriptase polymerase chain reaction （RT- PCR ） and enzyme-linked immunosorbent assay （ELISA）. Results After endothelial cells treated with 200 μmol·L-1 H2O2 for 24 h, the expression of P-selectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 mR- NA and protein level was significant higher. After TSG pretreatment endothelial cells for 4h before treated with 200 μmol·L-1 H2O2 results showed that TSG couldinhibit the expression of P-selectin, E-selectin, ICAM-1, VCAM-1 and MCP-1 mRNA and protein level induced by H2O2 on endothelial cells. Conclusion TSG can inhibit the expression of adhesion molecules induced by H2O2 on human umbilical vein endothelial cells and accordingly slow down atherosclerosis.

关 键 词：二苯乙烯苷 内皮细胞 动脉粥样硬化 Pselectin Eselectin ICAM1 VCAM1 MCP1 

分 类 号：R284.1[医药卫生—中药学] R322.12[医药卫生—中医学]