微量板倍量稀释法检测IgG抗A(B)效价改进研究  

Study of Modified Microplate Serial Twofold Dilution Test in Determining the Titer of Anti-IgG A/B Antibody

作　　者：陈海江[1] 丁杰锋[1] 

机构地区：[1]浙江省绍兴市妇幼保健院,312000

出　　处：《医学研究杂志》2012年第7期134-136,共3页Journal of Medical Research

摘　　要：目的通过实验研究改进微量板倍量稀释法检测IgG抗A(B)效价。方法采用微量板倍量稀释法,选取一个高值和一个低值标本来比较裂解液类型(2-Me、DTT)与稀释比例、裂解液处理时间和细胞温浴时间差异对结果的影响,综合得出最佳操作方案,将此方案与试管倍量稀释法进行比较,判断改进后的效果。结果得出改进后的操作方案:DTT与血清比例为3∶1,37℃处理时间为60min,细胞温浴时间为30min。与试管倍量稀释法比较无显著性差别(P>0.05)。结论改进后的方法是准确、有效的,更适合实验室进行大批量标本检测。Objective To modify microplate serial twofold dilution test in determining the titer of anti - IgG A/B antibody. Meth- ods We chose one high value and one low value samples to mensurate the titers in different lysis solution（2 - Me,DTT） , dilution ratios, lysis times, and inbubating times by microplate serial twofold dilution test. And we obtained an optimal modified method. Then the modi- fied method was compared with tube serial twofold dilution method. Results The optimal modified method was the follewing： the ratio of DTT to serum was 3 to 1 ;samples were treated at 37℃ in 60 minutes;cells incubated in 30 minutes. There was no significant difference （ P 〉 0.05 ） between two methods. Conclusion The modified method was accurate and effective, and it was more suitable for detecting a large number of samples on laboratory.

关 键 词：微量板倍量稀释法 试管倍量稀释法 效价 改进 

分 类 号：R722.17[医药卫生—儿科]