乙型肝炎病毒B／C基因型重组新方式的探讨  

A new recombination type of B/C genotype was discoved in hepatitis B virus

作　　者：高建梅[1] 董虹[1] 姚瑶[2] 王金丽[1] 沈涛[1] 邹云莲[1] 严新民[1] 

机构地区：[1]云南省第一人民医院临床基础医学研究所,昆明650032 [2]西双版纳州人民医院检验科

出　　处：《中华微生物学和免疫学杂志》2012年第5期410-414,共5页Chinese Journal of Microbiology and Immunology

基　　金：云南省卫生科技计划项目（2010NS004）

摘　　要：目的研究HBVB／C基因型重组方式。方法采用聚合酶链反应扩增4例景洪傻伲族慢性乙型肝炎患者血清中的HBV全基因并与pMDl8-T载体相连，转化人大肠杆菌E．coliJMl09中，经限制性内切酶酶切鉴定，阳性克隆DNA序列测定后进行HBV基因分型及重组部位的鉴定。结果4名患者16条HBV全基因均为有不同程度的C基因型毒株重组的B基因型，重组方式有两种：群1与C基因型毒株重组部位只有1个，位于HBV前C／C区ntl825-nt2320，共496bp；群2与C基因型毒株重组部位有2个，位于P基因区nt822-ntl020和前C／C基因区ntl825-nt2320，共695bp。结论这种B／C重组方式尚未见报道，Bj亚型与C基因型毒株除了在前C／C基因区重组外，同时还伴有少部分P基因区重组。Objective To study the recombination type of B/C genotype in hepatitis B virus. Methods The PCR was applied to amplifed the whole genes of HBV through the serums of four chronic HBV carriers who come from Jinghong distict, Yunnan province. The whole HBV genomes were ligated with pMD18-T vector and trasformed to E. coli JM109. After the positive colones were picked up, the HBV geno- types and reeombinated sites were diseoved through sequenced the acquired positive colones. Results All the acquired sixteen HBV sequences from the four HBV carriers were genotype B which were combinated with genotype C in some region. There are two ways of the combinations. For the first one, a 496 bp fragment from genotype C taked place the genotype B at the place of nt1825 to nt2320 of precore C/C region. For the second way, a 695 bp fragment of genotype B taked place at the both sites of nt822 to ntl020 of P gene region and nt1825 to nt2320 of precore C/C region. Conclusion A new recombination type of B/C genotype in hepatitis B virus was reported for the first time. The new Bj subgenotype was combinated with genotype C not only at the region of precore C/C but also at the place of P gene region.

关 键 词：乙型肝炎病毒 基因型 重组 

分 类 号：R51[医药卫生—内科学]