当归红芪超滤膜提取物对急性心肌缺血大鼠AST、LDH和LDH1的影响  

Effect of Ultra-filtration Extract Mixture from Angelica Sinensis and Radix Hedysari on AST,LDH and LDH1-Acute Myocardial Ischemia Rats

作　　者：陈雪娟[1] 倪志宏[1] 李应东[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中医药学报》2012年第3期33-35,共3页Acta Chinese Medicine and Pharmacology

基　　金：甘肃省自然科学基金项目(3ZS041-A25-064)

摘　　要：目的:探讨当归红芪超滤物对急性心肌梗死大鼠天冬氨酸氨基转移酶(aspartate aminotrans-ferase,AST),乳酸脱氢酶(actate dehydrogenase,LDH)和乳酸脱氢酶同工酶(lactate dehydrogenase,LDH1)活性的影响,阐明当归红芪超滤物对缺血心肌的保护作用。方法:结扎左前降支建立大鼠心梗模型。将80只雄性Wistar大鼠随机分成5组,每组16只,分别为造模组(myocardial infarction,MI)、假手术组(sham-operation,SH)、低剂量组(low-dose drug,LD)、中剂量组(middle dose group,MD)、高剂量组(high-dose drug,HD),分别检测给药24h和72h后外周血AST、LDH和LDH1的活性。结果:与SH组比较,MI组大鼠可见血清AST、LDH及LDH1活性明显增高(P<0.05);与MI组比较,药物各剂量组AST、LDH及LDH1活性明显降低(P<0.05),且峰值前移。结论:当归红芪超滤物可通过抗氧化作用降低AST、LDH及LDH1活性从而抗心肌缺血损伤,对大鼠心肌有明显的保护作用,且与用药时间及剂量密切相关。Objective ：To study the effect of ultra -filtration extract mixture from Angelica Sinensis and Radix Hedysari on activity of aminotransferase（ AST）, lactate dehydrogenase（LDH） and lactate dehydrogenase isoenzyme （ LDH1 ） in ser- um of acute myocardial ischemia rats. Methods ： Eighty healthy male wistar rats were randomly divided into five groups as Sham group, myocardial ischemia group, myocardial ischemia treated with low - dose, middle - dose and high - dose group; Rat myocardial ischemia model was established by ligation of the left anterior descending of coronary artery; the Angelica Sinensis and Radix Hedysari extract were obtained by ultrafiltration membrane technology ; the activity of AST, LDH and LDH1 in serum was detected by using spectrophotometry after treatment for 24h and 72h independently. Re- sults ： Compared with the sham group, the myocardial ischemia group showed that the activity of AST, LDI-I and LDH1 in serum was significantly increased（ P 〈 0. 05 ） ; the activity of AST, LDH and LDH1 in. serum for each myocardial ische- mia group treated with different doses of ultra - filtration extract mixture from Angelica Sinensis and Radix Hedysari was significantly decreased （P 〈 0. 05 ） compared with the myocardial ischemia group. Conclusion：The result shows that ultra -filtration extract mixture from Angelica Sinensis and Radix Hedysari can reduce the activity of AST, LDH and LDH1 in serum and have significantly protective effect on myocardial ischemia while showing a dose -effect and time -effect relationship.

关 键 词：当归红芪超滤物 急性心肌缺血 AST LDH LDH1 

分 类 号：R285.5[医药卫生—中药学]