抗痫灵对癫痫大鼠脑内nNOS活性影响的实验研究  

Effect of Kangxianling on the Expression of nNOS in Hippocampus of Epilepsy Rats

作　　者：孙丽[1,2] 冯铁为[3] 李敬孝[2] 

机构地区：[1]大庆龙南医院齐齐哈尔医学院附属第五医院,黑龙江大庆163458 [2]黑龙江中医药大学,黑龙江哈尔滨150040 [3]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药学报》2012年第3期36-38,共3页Acta Chinese Medicine and Pharmacology

摘　　要：目的:观察中药抗痫灵对戊四氮点燃癫痫大鼠模型的影响,进一步探讨从调节一氧化氮合酶(nNOS)的nNOS(神经元型)亚型代谢的角度探讨抗痫灵的抗癫痫作用机制。方法:动物模型采用戊四氮点燃大鼠模型。免疫印迹(Western blot)方法观察实验各组大鼠大脑海马nNOS的活性变化。结果:抗痫灵组、丙戊酸钠组、模型组阳性细胞表达与正常对照组相比,有显著性差异(P<0.05),模型组较正常对照组大鼠脑nNOS内活性显著升高(P<0.01),中药组显著低于模型组(P<0.01)。结论:抗痫灵对戊四氮点燃大鼠癫痫发作有显著对抗作用,其作用机制可能与其降低模型大鼠脑内nNOS活性有关。Objective：To observe the effect of Kangxianling on the expression of nNOS in hippocampus of the PTZ - in- duced epilepsy model of rats, we probed into the efficacy and the mechanism of Kangxianling. Methods ： PTZ was used to evoke chronic epilepsy model. Ethology changes and electroencephalography were observed after epileptic seizure, expres- sions of nNOS were investigated with Western blot method. Results：The groups of Kanxianling, VPA groups and PTZ- groups were significantly different from the control group （ P 〈 0. 05 ）. The groups of Kanxianling was siginificantly differ- ent from the model group（ P 〈0.01 ）. Conclusion ： Kangxianling has anti -epileptic seizure resistant function on PTZ - induced epilepsymodel of rats. Its function mechanism may be to react by reducing the content of nNOS in the hippocampus.

关 键 词：癫痫 抗痫灵 戊四氮(PTZ) 神经型一氧化氮合酶(nNOS) 

分 类 号：R285.5[医药卫生—中药学]