脊髓背角P物质在电针调控炎性痛大鼠神经-免疫网络中的作用研究  

Involvement of Substance P in Inflammmatory Pain and Electroacupuncture Regulating Network of Immune-neuroendocrine Interactions

作　　者：王振宇[1] 李淑莲[1] 王希文[1] 孙忠人[1] 

机构地区：[1]黑龙江中医药大学黑龙江省普通高校重点实验室针灸临床神经生物学实验室,黑龙江哈尔滨150001

出　　处：《中医药学报》2012年第3期88-90,共3页Acta Chinese Medicine and Pharmacology

基　　金：教育部春晖计划课题(Z2009-1-15027);黑龙江中医药大学博士创新基金(B201004);黑龙江省教育厅面上项目(12511505)

摘　　要：目的:通过阐明在脊髓背角疼痛信号传输中的关键神经肽SP在电针夹脊穴治疗大鼠单发关节炎中的作用,揭示夹脊电针抑制炎性痛的神经-免疫机制。方法:随机数字表法将大鼠分为假造模组、模型组、西乐葆组、电针组、电针+西乐葆组,采用弗氏完全佐剂建立的单侧足底慢性炎症痛大鼠模型,采用实时定量PCR法检测大鼠脊髓中SP mRNA在电针夹脊穴治疗大鼠单发关节炎中过程中的变化。结果:各组单侧足底慢性炎症痛大鼠模型脊髓中的SP mRNA表达与假造模组比较显著性增加(P<0.001);电针夹脊穴组大鼠SP mRNA的表达与模型组比较明显降低(P<0.01),且电针夹脊穴增强灌饲西乐葆对炎性痛大鼠脊髓中SP mRNA表达的抑制作用(P<0.01)。结论:电针通过下调炎性痛大鼠神经-免疫网络环路中的重要神经肽脊髓背角SP的表达,从而发挥镇痛作用。Objective ： To reveal the mechanism of involvement of spinal cord pain control system SP in inflammatory pain and the regulation of network of immune - neuroendocrine interactions by electroacupuncture （EA）. Methods ： The rats were randomly divided into sham surgery group, model group, Celebre group, EA group, EA plus Celebre group. Monoar- thritis （MA） was induced by an injection of complete Freund＇ s adjuvant （CFA）. The present study was designed to ex- plore the changes mRNA levels of SP in spinal cord of MA rats and EA analgesia by using Real - time PCR. Results ：The mRNA levels detected by Real - time PCR of SP in spinal cord in EA group were significantly decreaced as compared to those in MA group （ P 〈 0.01 ） ;Combination of EA with Celebre significantly enhanced the inhibitory effects （ P 〈 0.01 ）. Conclusion：Celebre and EA synergistically block activation of spinal microglia and subsequent astrocytes can reduce the release of SP in the spinal cord, thereby leading to a reinforcing inhibitory effect on MA - induced thermal hyperalgesia.

关 键 词：夹脊电针 神经-免疫网络 单侧炎症痛 镇痛 P物质 

分 类 号：R245.97[医药卫生—针灸推拿学]