HPLC法同时测定清热灵颗粒中黄芩苷和连翘苷含量  

Determination of the Content of Baicalin and Forsythin in Qingreling Granules by HPLC

作　　者：焦付哲 

机构地区：[1]南阳市卫生职工学校,河南南阳473000

出　　处：《黑龙江医药》2012年第3期344-345,共2页Heilongjiang Medicine journal

摘　　要：目的:建立同时测定清热灵颗粒中黄芩苷和连翘苷含量的HPLC方法。方法:色谱柱:Kromasil C18(250mm×4.6mm,5μm)。流动相:乙腈-0.5%三乙胺水溶液(磷酸调pH至3.05)(30:70)。检测波长为277nm,流速:1.0ml·min-1;柱温:25℃。结果:黄芩苷线性范围为1.65～105.57μg·ml-1(r=0.9999),平均回收率为98.94%,RSD为0.39%(n=6);连翘苷线性范围为1.63～104.55μg·ml-1(r=0.9999),平均回收率为99.00%,RSD为0.46%(n=6)。结论:方法简便可靠,结果重现性好,为控制清热灵颗粒的内在质量提供了科学依据。Objective ：To establish a method for determination of the content of baicalin and forsythin in Qingreling Gran- ules by HPLC. Methods：The Kromasll Cls column （250 mm×4. 6 mm ,5μm）was used,The mobile phase was aeetonitrile - 0. 5% trlethylamine（adjust pH to 3.05 with phosphoric acid） （30：70）. The detection wavelength was at 277 nm,the flow rate was 1.0 ml·min -l . The temperature of the column at 25℃. Results： the linear range for baicalin and forsythin was 1.65-105.57 μg·ml-1 （ r = 0. 9999） and1.63-104. 55 μg·nd - 1（ r=0. 9999 ） , the average recovery was 98.94% （ RSD 0. 39%, n = 6 ） and 99.00% （ RSD0. 46%, n = 6）. Conclusion： Tile method is simple, accurate, reliable and can be applied to the quality control of Qingreling Granules.

关 键 词：清热灵颗粒 黄芩苷 连翘苷 HPLC法 

分 类 号：R927.2[医药卫生—药学]