秦皮乙素诱导人胃癌SGC-7901细胞凋亡机制的研究  

Study on the Mechanism of Esculetin-induced Apoptosis in SGC-7901 Human Gastric Cancer Cells

作　　者：贾绍华[1,2,3] 刘冰洁[3] 张道勇[3] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [3]哈尔滨商业大学药学院,哈尔滨150076

出　　处：《黑龙江医药》2012年第3期365-368,共4页Heilongjiang Medicine journal

摘　　要：目的:研究秦皮乙素对SGC-7901细胞的作用及其作用机制。方法:首先通过MTT法考察秦皮乙素对胃癌SGC-7901细胞体外抑瘤作用,并用电镜法观察经不同浓度的秦皮乙素作用的SGC-7901细胞的形态特征,最后用流式细胞仪法检测bcl-2、bax蛋白的表达。结果:秦皮乙素具有一定的抗肿瘤作用,随着给药浓度的增加,SGC-7901细胞的生长率也随之降低,且有明显的凋亡形态学特征。药物作用24h后,用FCM法检测到bcl-2的表达随给药浓度的增加而降低,而bax表达呈上升趋势。结论:秦皮乙素对SGC-7901细胞有显著的抑制作用且诱导其凋亡。Objective：To investigate the effect and its mechanism of esculetin on SGC -7901 cells. Methods：At first,in- vestigating the effect of esculetin on SGC -7901 cells by MTr method, and then observing the morphological feature changes of apoptosis cells with esculetin of different concentration by transmission electron microscope, at last analyzing the protein lev- els of bax, bcl -2 in SGC -7901 cell line using FCM method. Results：Eseuletin significantly inhibited the growth of SGC - 7901 cells and gready decreased the growth rate of SGC -7901 cells with the eseuletin concentration increased,and then had observed the morphological feature changes of apoptosis cells. After 24h, esculetin down - regulated the expression of Bcl - 2 protein and up -regulated the expression of Bax protein by using FCM method. Conclusion ：The study showed that eseuletin remarkably inhibit the growth of SGC -7901 cells and induce apoptosis of SGC- 7901 cells.

关 键 词：秦皮乙素 SGC-7901细胞 细胞凋亡 作用机制 

分 类 号：R285[医药卫生—中药学]