绵羊肺腺瘤病毒囊膜蛋白在大鼠体内的表达  

Expression of OPAV-Env Protein in Rats

作　　者：李靖[1] 瓦西力[1] 周艳喜[1] 么宏强[1] 于立新[1] 敖威华 马学恩[1] 

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010018 [2]内蒙古出入境检验检疫局技术中心,呼和浩特010020

出　　处：《动物医学进展》2012年第7期7-11,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31060332)

摘　　要：为探讨绵羊肺腺瘤病毒囊膜蛋白(OPAV-Env)引起肺泡Ⅱ型上皮恶变的机理,将真核表达质粒pcDNA3.1-env用体内转染试剂经尾静脉转染到幼龄大鼠体内,用RT-PCR及免疫组化法检测绵羊肺腺瘤病毒囊膜蛋白在大鼠体内的表达,并采用SSCP法及基因克隆法结合序列分析检测H-ras基因是否发生突变。结果显示,绵羊肺腺瘤病毒囊膜蛋白在大鼠肺脏组织存在表达,但转染21d后和正常大鼠比较,未发现大鼠H-ras基因发生突变。To explore the mechanism of alveolar type Ⅱ cell malignant transformation which was caused by the OPAV-env gene, the eukaryotic expression plasmid pcDNA3. 1-env was transfected into young rats with vivo transfection reagent through tail vein injection method. Then the RT-PCR and immunohisto- chemistry were used to detect the expression of pcDNA3.1-env plasmid in rats. Mutations of ras gene was detected by using methods of SSCP, gene clone and sequence analysis. The results showed that the plasmid pcDNA3.1-env was expressed in lung tissue of rats. 21 days after transfection, the ras gene mutation was not discovered compared with normal rats.

关 键 词：绵羊肺腺瘤病毒 RAS基因 免疫组化 单链构象多态性 

分 类 号：Q786[生物学—分子生物学] S852.659.3[农业科学—基础兽医学]