银中杨微体繁殖技术研究  

Study on Microbody Propagation of Populus alba×P.berolinensis

作　　者：姜长吉 姜辉 程广有[3] 

机构地区：[1]吉林市龙潭区江密峰苗圃,吉林吉林132206 [2]吉林市龙潭区金珠林业站,吉林吉林132104 [3]北华大学林学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2012年第3期335-338,共4页Journal of Beihua University（Natural Science）

摘　　要：利用MS培养基加入不同质量浓度的6-苄基氨基腺嘌呤（6-BA）和萘乙酸（NAA）进行银中杨微体繁殖技术研究.结果表明：以银中杨带芽枝条为外植体可以实现微体繁殖,适宜的表面消毒时间为8 min.MS培养基中6-BA含量较高时侧芽发生时间缩短、数量增加,侧芽增殖的培养基配方以MS＋6-BA 1.5 mg/L＋NAA 0.05 mg/L效果较好;银中杨试管苗在1/2 MS＋1.0 NAA中生根率为86%,根系发达;银中杨试管苗在以珍珠岩＋蛭石的基质中成活率最高（98%）,在旱田土＋珍珠岩（1 1）基质中成活率次之（76%）,在旱田土＋蛭石（1 1）基质中成活率最低（68%）.The technology of microbody propagation of Populus alba x P. berolinensis was studied in MS medium plus 6-benzyladenine （6-BA） and naphthalene acetic acid （NAA）. the results showed：The explant which was stem piece with bud of Populus alba x P. berolinensis was good for propagation and it was good time for explant surface sterilization 8 min. There were more lateral buds in shot time if putting more 6-benzyladenine （6-BA） in MS medium. The good for latural bud propagation medium was MS ＋ 6-BA1.5mg/L ＋ NAA0.05mg/L. The good medium to induce root of Populus alba x P. berolinensis was 1/2MS ＋ NAA1.0mg/L（86% rooted ratio） ,and its system is good in the medium. There was higher proportion of viability planted test-tube seedlings in the mixed substratum （ pearlite and vermiculite, 1 ： 1 ）. The proportion of viability planted test-tube seedlings was 76% and 68 % in the dry farmland soil plus pearlite （ 1 ： 1 ） and dry farmland soil plus vermiculite （ 1 ： 1 ）, separately.

关 键 词：银中杨 微体繁殖 移栽 

分 类 号：Q813.12[生物学—生物工程] S723.132[农业科学—林木遗传育种]