2010年泰安市手足口病病原学检测分析  

Analysis on the pathogenic diagnosis of the hand-foot-mouth diseases in Taian in 2010

作　　者：侯佩强[1] 张荣强[1] 倪楠[1] 

机构地区：[1]山东省泰安市疾病预防控制中心,泰安市271000

出　　处：《中华实验和临床感染病杂志（电子版）》2012年第3期30-32,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：泰安市科学技术发展计划项目(No.20103020)

摘　　要：目的对2010年泰安市手足口病病例进行病原体检测和分析。方法对临床收集的手足口病病例的粪便标本437份、咽拭子标本25份,采用实时荧光PCR筛选出肠道病毒通用引物阳性标本,分别用CA16和EV71型的特异引物进行分类检测。结果肠道病毒总阳性率为82.47%(381/462),其中CA16阳性率为38.31%(177//462),EV71阳性率为19.48%(90/462),CA16的阳性率显著高于EV71。粪便标本的阳性率为84.67%(370/437),显著高于咽拭子的44%(11/25)(χ2=27.05,P<0.01)。本研究病例发病年龄多为1～5岁,男性略多于女性。结论 2010年度泰安市手足口病疫情以CA16感染为主,粪便标本的阳性率较高,采用分子生物学方法检测手足口病病原体对手足口病的监测及防控具有重要意义。Objective To analyze the pathogenic diagnosis of hand-foot-mouth diseases （HFMD） in Taian in 2010. Methods Total of 437 feces specimens and 25 throat swabs specimens from clinical-diagnosed HFMD patients were collected. Pan-enterovirus was detected by real-time flueorecent PCR,and Coxsackie A16 （CA16） and Enterovirus 71 （EV71） were detemined by species-special primers. Results Positive rate of Pan-enterovirus, CA16 and EV71 were 82.47% （381/462）, 38.31% （177/462） and 19.48% （90/462）, respectively. The positive rate of CA16 was significantly higher than that of EV71. positive rate of feces specimens（84.67%, 370/437） was significantly higher than that of throat swabs specimens （44%, 11/25） （x2 = 27.05,P〈 0.01）. The age of onset was mostly from 1 to 5 years. The prevalence rate of male was higher than that of female. Conclusions CA16 was the dominant pathogen of the HFMD in Taian in 2010. The feces specimens have a higher positive rate. Molecular biological methods on pathogenic diagnosis plays an important role in the surveillance and control of HFMD.

关 键 词：手足口病 肠道病毒 EV71 CA16 实时荧光PCR 

分 类 号：R725.1[医药卫生—儿科]