单分子力谱研究活细胞上多药耐药相关蛋白1的表达  

Investigation of MRP1 Molecules on Cell Membrane Based on Single Molecule Atomic Force Microscopy

作　　者：王青[1] 孙小兰[1] 羊小海[1] 王柯敏[1] 吴春玲[1] 陈桐[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,化学化工学院,生物纳米与分子工程湖南省重点实验室,长沙410082

出　　处：《高等学校化学学报》2012年第7期1401-1406,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:90606003;20805012);国家"九七三"计划项目(批准号:2011CB911002);科技部国际合作重大项目(批准号:2010DFB30300);教育部"新世纪优秀人才支持计划"(批准号:NCET-09-0338);湖南省高等学校青年骨干教师培养对象基金(2009年度)资助

摘　　要：采用原子力显微镜的单分子力谱(SMFM)技术研究了多药耐药相关蛋白1(MRP1)与其抗体间的相互作用,并考察了人舌癌细胞系TCA8113经高剂量平阳霉素(BLM)反复间歇诱导前后细胞表面MRP1的表达差异.实验结果表明,MRP1与其抗体之间存在特异性相互作用力,当针尖运动速率为2.5μm/s时,作用力大小约为(182±35)pN;而且药物诱导后MRP1在人舌癌细胞上的表达明显增强.本工作为了解活细胞水平上MRP1的表达提供了新方法,有助于肿瘤细胞多药耐药性(MDR)的研究.Multidrug resistance（MDR） is one of the main factors in the failure of chemotherapy against tumors,and multidrug resistance-associated protein 1（MRP1） is closely related to the generation of MDR.In this work,the interaction force between MRP1 and anti-MRP1 was measured via single-molecule force microscopy（SMFM）,and the expression differences of MRP1 on human tongue cancer cells before and after treatment with high-dose bleomycin（BLM） were investigated.The results show that the interaction force between MRP1 and anti-MRP1 is about（182±35） pN as the loading rate is 2.5 μm/s.The expression of MRP1 on human tongue cancer cell membrane increased obviously after treatment with high-dose BLM.This work may provide a new method for studying the expression of MRP1 at the living cell level and can be helpful for the study of MDR.

关 键 词：原子力显微镜 单分子力谱 多药耐药性 多药耐药相关蛋白1(MRP1)表达 

分 类 号：O657[理学—分析化学]