基于双纳米金探针杂交法检测HBV DNA  

Detection of Hepatitis B Virus DNA Hybridization Based on Dual Gold Nano-probes

作　　者：邢亚斯[1,2] 邹能利[1] 毛红菊[1] 徐霞[2] 葛玉卿[1] 金庆辉[1] 赵建龙[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所传感技术联合国家重点实验室,上海200050 [2]郑州大学药学院,郑州450001

出　　处：《高等学校化学学报》2012年第7期1420-1425,共6页Chemical Journal of Chinese Universities

基　　金：上海市科学技术委员会纳米专项(批准号:11nm0505800;1052nm061000);国家自然科学基金(批准号:31000791)资助

摘　　要：利用双纳米金探针结合基因芯片平台建立了一种检测乙肝病毒基因(HBV DNA)的新方法.根据HBVDNA的保守序列设计捕获探针和信号报告探针,通过一对互补的纳米金检测探针的双杂交法对HBV DNA进行信号放大,最后进行银染,达到对HBV DNA的可视化检测.该方法的灵敏度高,可检测10 fmol/L的HBV DNA,且能在1.5 h内完成检测.其具有的快速、高灵敏度及低成本等优势使其有望发展成为一种检测HBV DNA的新方法.A novel Hepatitis B virus（HBV） DNA detection method based on dual gold nano-probes and gene chip was developed.Two types of probes including capture probe and reporter probe were designed according to conservative HBV DNA.A pair of complementary gold nanoparticles probes was used to amplify the signal of HBV DNA and visualized by silver staining on gene chip.The optimized detection limit of this method was 10 fmol/L,and the entire test could be completed in 1.5 h.This strategy could be potentially used for HBV DNA detection due to its rapid,high-sensitivity and low-cost properties.

关 键 词：双纳米金探针 杂交 基因芯片 银染 乙肝病毒基因(HBV DNA) 

分 类 号：O657.39[理学—分析化学]