杜氏盐藻核基质结合区结合蛋白的表达分析  

Analysis of Expression of Matrix Attachment Region Binding Protein from Dunaliella Salina

作　　者：王鹏举[1] 王天云[2] 王亚峰[3] 

机构地区：[1]郑州大学基础医学院,河南郑州450001 [2]新乡医学院生物化学与分子生物学教研室,河南新乡453003 [3]郑州大学药学院,河南郑州450001

出　　处：《河南科技大学学报（医学版）》2012年第2期81-84,共4页Journal of Henan University of Science & Technology:Medical Science

基　　金：国家自然科学基金项目(30970055)

摘　　要：目的研究杜氏盐藻(Dunaliella salina)核基质结合区结合蛋白(matrix attachment region bindingprotein,MBP)基因随盐藻的生长表达变化情况及真核表达。方法利用实时荧光定量PCR检测盐藻中MBP基因的表达变化,构建MBP的cDNA全长序列及N端和C端序列的含有组氨酸标签的真核表达载体,转染CHO细胞,筛选稳定转染株,Western blotting检测融合蛋白表达情况。结果盐藻的MBP基因表达随生长周期变化,MBP及其N端和C端的融合蛋白成功在CHO细胞表达。结论 MBP可能参与盐藻增殖过程中RNA的加工,并为下一步分离真核表达的MBP,研究其功能提供了实验基础。Objective To analyze expression of matrix attachment region binding protein （MBP） along with the growth of Dunaliella salina, and investigate eukaryotie expressioh of MBP. Methods Real-time PCR was performed to analyze the expression of MBP in Dunaliella salina,~ and full length, N-terminal and C- terminal of MBP gene of Dunaliella salina were amplified and fused to the His-tag to construct the eukaryotic vectors. Then, the recombinant vectors were transfected into Chinese hamster ovary （CHO） cells by Lipofectamine 2000. Western blotting was performed to determine the expression of the fusion proteins. Results Expression of MBP gene fluctuated in the growth cycle of Dunaliella salina, the fusion proteins were expressed in CHO cells. Conclusion The findings mentioned above suggest that MBP may be involved in RNA processing of the proliferation of Dunaliella salina, and contribute to further investigation of the function of MBP.

关 键 词：杜氏盐藻 核基质结合区结合蛋白 真核表达 

分 类 号：Q74[生物学—分子生物学]