人工冬虫夏草在大鼠慢性阻塞性肺疾病中的治疗作用和机制研究  

Study on the therapeutic effect and its mechanism of Cordyceps sinensis cultivated on rats＇ chronic obstructive pulmonary disease

作　　者：廖东江[1] 卢心鹏[1] 赵瑾[1] 刘蓉[1] 赵红波[1] 李洪涛[1] 莫自耀[1] 

机构地区：[1]呼吸疾病国家重点实验室(广州医学院) 广州医学院第一附属医院 广州呼吸疾病研究所,510120

出　　处：《国际呼吸杂志》2012年第13期969-973,F0003,共6页International Journal of Respiration

摘　　要：目的研究人工冬虫夏草对香烟烟雾所致慢性阻塞性肺疾病（COPD）大鼠模型的治疗和作用机制。方法SD健康雄性大鼠随机分为对照组、COPD组、造模前给药组、造模后给药组、中剂量（2．5g·kg-1·d-1）和高剂量（5g·kg-1·d-1）治疗组共6组，每组10只。动物连续烟熏90d造模。造模前给药组于造模前两周开始给药，治疗组在造模前给药的基础上持续给药直至造模结束。造模后给药组为在造模结束后给药两周。测定各组大鼠肺功能，观察肺组织病理学变化，提取肺组织总蛋白进行双向电泳，对表达差异显著的蛋白点进行鉴定分析。结果气道阻力均值COPD组（O．3869cmH2O·ml-1·s）明显高于对照组（0．2630cmH20·ml-1·s）（P〈0．05）；造模前给药组（0．2525cmH2O·ml-1·s）、中剂量治疗组（O．2233cmH2O·ml-1·s）、高剂量治疗组（0．1846cmH2O·ml-1·s）、造模后给药组（O．3011cmH2O·ml-1·s）均明显低于COPD组（P〈O．05），与对照组相比差异无统计学意义。给药各组特别是高剂量组与COPD组相比支气管和肺部病理情况均有明显改善，结果差异有统计学意义（P〈O．05）。与对照组相比有14种蛋白质的表达量在COPD组中显著下降。谷胱甘肽过氧化物酶、醛脱氢酶及线粒体醛脱氢酶前体等三种蛋白则在高剂量治疗组中表达量得到恢复和改善。结论人工冬虫夏草对大鼠cOPD有明确的治疗作用，推测与抑制或改善肺部组织氧化与抗氧化失衡以及改善全身状况有关。Objective To investigate the therapeutic effect of cultivated Cordyceps sinensis on rats＇s chronic obstructive pulmonary disease （COPD） by inhaling cigarette and analyze on the mechanism. Methods Ten-week-old male rats were selected and divided randomly into 6 groups as control group, COPD group, pre-administrated group, post-administrated group, moderate dosage group and high dosage group. Each group has 10 animals. The experimental rats were fumigated with simple smoking exposure for 90 days to establish a COPD model. Pre-administrated group were treated with Cordyceps sinensis for two weeks before fumigation. Besides receiving two weeks＇ drug before fumigation, moderate dosage （2.5 g kg-1 d-1） and high dosage （5 g kg d-1） were given drug for the whole fumigation period. Post-administrated group received Cordyceps ＂sinertsis for two weeks after fumigation. The animals＇ pulmonary function and lung tissue pathological changes were examined and observed. The total proteins from lung tissue were collected and separated by 2-dimensional electrophosis. The different significant expression proteins were identified by MS and analyzed with NCBI data. Results The average value of airway resistance of COPD group （0. 386 9 cm H2O·ml-1· s） is higher significantly than that of control group （0. 263 0 cm H2O·ml-1· s） （ P d0.05）. The values from pre-administrated group （0. 252 5 cm H2O·ml-1· s）, moderate dosage group （0. 223 3 cm H2O·ml-1· s）, high dosage group （0. 184 6 cm H2O·ml-1· s） and post-administrated group （0. 301 1 cm H2O·ml-1· s）,is lower significantly than that of COPD group （all P 〈0.05）. But the values from the 4 drug-treatment groups have no statistical difference when compared with that of the control group. Statistically, the pulmonary functions and pathological conditions of the treatment groups, especially high dosage group, were improved greatly when compared with that of COPD group were improved significantly. There are 14 lung tissue

关 键 词：人工冬虫夏草 大鼠 慢性阻塞性肺疾病 治疗 机制 

分 类 号：R285.5[医药卫生—中药学]