抑郁大鼠脑内NMDA受体NR1、NR2B表达及中药干预作用  

Expressions of brain NR1 and NR2B of NMDA receptor in depressive rats and interference effect of Chinese medicinals

作　　者：赵瑞珍[1] 唐启盛[1] 田青[1] 李小黎[1] 

机构地区：[1]北京中医药大学第三附属医院,北京100029

出　　处：《北京中医药大学学报》2012年第6期383-385,390,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局行业科研专项经费资助项目(No.200807027);国家自然科学基金资助项目(No.30973740)

摘　　要：目的研究抑郁大鼠脑内N-甲基-D-天冬氨酸(NMDA)受体亚单位NR1mRNA、NR2BmRNA的表达及中药颐脑解郁方的干预作用。方法二级雄性大鼠分为正常组、抑郁组、治疗组、对照组,后3组采用慢性应激方法结合孤养法建立抑郁模型,治疗结束后,取脑组织,采用逆转录PCR(RT-PCR)实验方法,研究NR1mRNA、NR2BmRNA在抑郁模型大鼠脑内左前额皮质、海马的表达及中药颐脑解郁方的干预作用。结果抑郁组大鼠海马、前额皮质的NR1、NR2B表达明显高于正常组,中药颐脑解郁方干预后可明显降低NR1mRNA、NR2BmRNA在脑内的表达。结论抑郁模型大鼠脑内NR1mRNA、NR2BmRNA表达升高,中药颐脑解郁方可使之明显下调,中药颐脑解郁方的抗抑郁作用可能与下调NMDA受体(NMDAR)的表达有关。Objective To study the expressions of brain receptor subunits of N-methyl-D-aspartic acid （NMDA） -NRI mRNA and NR2B mRNA in depressive rats and interference effect of Yinao Jieyu Fang. Methods Male Wistar rats were divided into normal group, depression group, treatment group and control group. The depression model was established by using chronic stress and independent feeding in the depression group, treatment group and control group. After the end of treatment, the brain samples were collected from rats. The expressions of NR1 mRNA and NR2B mRNA in rat left frontal cortex and hippocampus, and the interference effect of Yinao Jieyu Fang were studied by using RT-PCR. Results In depression group, the expressions of NR1 mRNA and NR2B mRNA in rat left frontal cortex and hippocampus were significantly higher than those in normal group. After the interference of Yinao Jieyu Fang these expressions decreased significantly. Conclusion The expressions of NR1 mRNA and NR2B mRNA increase in depressive rats and Yinao Jieyu Fang can down-regulate them significantly. The anti-depression effect of Yinao Jieyu Fang may be related to the down-regulation of NMDAR expression.

关 键 词：抑郁 颐脑解郁方 N-甲基-D-天冬氨酸 N-甲基-D-天冬氨酸受体1 N-甲基-D-天冬氨酸受体2B 大鼠 

分 类 号：R285.5[医药卫生—中药学]