SSR分子标记技术在遗传学实验教学中的应用  

Application of SSR marker in genetics experimental teaching

作　　者：夏曦中[1] 车婧[1] 章志宏[1] 王建波[1] 

机构地区：[1]武汉大学生命科学学院,湖北武汉430072

出　　处：《实验技术与管理》2012年第6期48-50,共3页Experimental Technology and Management

基　　金：湖北省高等学校教学研究项目(2009002)

摘　　要：该实验是科研成果转化为实验教学的一个案例。选择位于水稻不同连锁群上的6对SSR引物构建了2个杂交稻及其亲本的SSR指纹图谱,建立了一套适合于本科生实验的杂交水稻亲本鉴定的稳定的SSR技术体系。筛选的6对引物进行PCR扩增得到的杂交稻均有2条带。由SSR指纹图谱分析可得:杂交稻P5的亲本是P2和P4,P6的亲本是P1和P3。通过本实验巩固了学生关于分离定律的学习,并有所延伸。This is a case to turn an achievement in scientific research into the teaching experiment.It helps students to learn the theory and method of SSR marker from the experiment,and helps them grasp the technology how to identify the genetic relatives based on SSR fingerprint.It is adapted to teaching experiment that the technology of the six SSR primers in different chromosomes in hybrid rice is selected to distribute the SSR fingerprint of two hybrid rice combinations and their parents.The two bands of hybrid rice are complement types of their parents.Based on the SSR fingerprint,it can conclude that P2 and P4 are the parents of P5,and P1 and P3 are the parents of P6.Mendel＇s law of segragation and other knowledge can be consolidated by this experiment.

关 键 词：遗传标记 遗传学实验 SSR 杂交水稻 

分 类 号：Q33[生物学—遗传学] G642.423[文化科学—高等教育学]