臭氧对水稻叶片膜脂过氧化和抗氧化系统的影响  

Effects of Ozone on Membrane Lipid Peroxidation and Antioxidant System of Rice Leaves

作　　者：金明红[1] 冯宗炜[1] 张福珠[1] 

机构地区：[1]中国科学院生态环境研究中心,北京100080

出　　处：《环境科学》2000年第3期1-5,共5页Environmental Science

基　　金：国家自然科学基金!资助项目 ( Project Supported by theNational Natural Science Foundation of China) :498992 70

摘　　要：采用 OTC- 1型开顶式气室研究臭氧对不同生育时期水稻叶片膜脂过氧化和抗氧化系统的影响 .实验结果表明 ,随着臭氧浓度增加 ,水稻叶片叶绿素含量、叶绿素 a/b值下降 ,膜相对透性和丙二醛 (MDA)含量上升 ,过氧化物酶 (POD)活性逐渐上升 ,过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)活性开始被诱导上升 ,当浓度达到 2 0 0 nl· L- 1时反而下降 .叶绿素含量及叶绿素a/b值与 MDA含量呈显著负相关 ,膜透性与 MDA含量呈显著正相关 ,叶绿素含量与 POD活性呈负相关 .臭氧造成活性氧的产生和清除之间的失衡 ,加剧了膜脂过氧化作用 ,对水稻的膜系统产生了危害 ,降解叶绿素 ,加速叶片的老化 .Rice plants were used to investigate effects of ozone on membrane lipid peroxidation and antioxidant system of rice leaves in varied growth stage.The plants were exposed in open top chambers (OTCs)in field conditions to four levels of O 3 concentration (charcoal filtered air,50nl·L -1 ,100nl·L -1 ,200nl·L -1 )from 1999 07 04 to 1999 10 01.With O 3 concentration increasing,chlorophyll content and chlorophyll a/b in rice leaves were significantly declined;membrane permeability and the level of lipid peroxidation (MDA) content were significantly increased;the activity of CAT,SOD increased first and decreased afterward;the activity of POD gradually increased.Chlorophyll content was negatively correlated with MDA content and membrane permeability was positively correlated with MDA content.Those observations indicated the O 3 stress induce in vivo oxidative injury and affect the activities of antioxidant enzymes.The effects resulted in imbalance of activated oxygen produce and scavenge,accelerated process of lipid peroxidation,imposed on the rice leaves detrimentally.

关 键 词：臭氧 膜脂过氧化 抗氧化系统 水稻 叶片 

分 类 号：X511.032.3[环境科学与工程—环境工程]