Methanogenic community dynamics in anaerobic co-digestion of fruit and vegetable waste and food waste  

作　　者：Jia Lin Jiane Zuo Ruofan Ji Xiaojie Chen Fenglin Liu Kaijun Wang Yunfeng Yang 

机构地区：[1]State Key Joint Laboratory of Environment Simulation and Pollution Control(SKLESPC),School of Environment,Tsinghua University,Beijing 100084,China

出　　处：《Journal of Environmental Sciences》2012年第7期1288-1294,共7页环境科学学报（英文版）

基　　金：supported by the Ministry of Science and Technology of China(No.2008BADC4B16,2008BADC4B18,2008AA062401)

摘　　要：A lab-scale continuously-stirred tank reactor （CSTR）, used for anaerobic co-digestion of fruit and vegetable waste （FVW） and food waste （FW） at different mixture ratios, was operated for 178 days at the organic loading rate of 3 kg VS （volatile solids）/（m3.day）. The dynamics of the Archaeal community and the correlations between environmental variables and methanogenic community structure were analyzed by polymerase chain reactions - denaturing gradient gel electrophoresis （PCR-DGGE） and redundancy analysis （RDA）, respectively. PCR-DGGE results demonstrated that the mixture ratio of FVW to FW altered the community composition of Archaea. As the FVW]FW ratio increased, Methanoculleus, Methanosaeta and Methanosarcina became the predominant methanogens in the community. Redundancy analysis results indicated that the shift of the methanogenic community was significantly correlated with the composition of acidogenic products and methane production yield. Different mixture ratios of substrates led to different compositions of intermediate metabolites, which may affect the methanogenic community. These results suggested that the analysis of microbial communities could be used to diagnose anaerobic processes.A lab-scale continuously-stirred tank reactor （CSTR）, used for anaerobic co-digestion of fruit and vegetable waste （FVW） and food waste （FW） at different mixture ratios, was operated for 178 days at the organic loading rate of 3 kg VS （volatile solids）/（m3.day）. The dynamics of the Archaeal community and the correlations between environmental variables and methanogenic community structure were analyzed by polymerase chain reactions - denaturing gradient gel electrophoresis （PCR-DGGE） and redundancy analysis （RDA）, respectively. PCR-DGGE results demonstrated that the mixture ratio of FVW to FW altered the community composition of Archaea. As the FVW]FW ratio increased, Methanoculleus, Methanosaeta and Methanosarcina became the predominant methanogens in the community. Redundancy analysis results indicated that the shift of the methanogenic community was significantly correlated with the composition of acidogenic products and methane production yield. Different mixture ratios of substrates led to different compositions of intermediate metabolites, which may affect the methanogenic community. These results suggested that the analysis of microbial communities could be used to diagnose anaerobic processes.

关 键 词：high-solid organic waste anaerobic co-digestion methanogenic community structure denaturing gradient gel elec-trophoresis （DGGE） redundancy analysis （RDA） 

分 类 号：X511[环境科学与工程—环境工程] X712