葡萄籽原花青素对环磷酰胺诱发小鼠骨髓多染红细胞微核形成的抑制作用及其机理探讨  

The Inhibitory Eeffect and its Mechanism Procyanidins from Vitis vinifera Seed on Cyclophosphamide Inducing Micronuclear Formation

作　　者：孙志广[1] 赵万洲[2] 陆茵[2] 唐玲芳[3] 张珍玲[3] 阮君山[2] 闫新琦 薛普凤 张世玮[2] 

机构地区：[1]江苏省中医院,江苏南京210029 [2]南京中医药大学,江苏南京210029 [3]南京医科大学,江苏南京210029

出　　处：《时珍国医国药》2000年第5期386-387,共2页Lishizhen Medicine and Materia Medica Research

基　　金：中国博士后基金资助项目! No.1997.7

摘　　要：通过微核试验和小鼠肝组织中谷胱甘肽 -S-转移酶 ( GST)活性、还原型谷胱甘肽 ( GSH )含量的测定 ,在哺乳动物整体水平 ,从遗传学和生化毒理学角度进一步研究了原花青素 ( PV2 0 0 0 )的抗诱变作用及其机理。结果发现 ,原花青素对环磷酰胺 ( CPA)诱发的小鼠骨髓多染红细胞 ( PCEs)微核细胞率有较强抑制作用 ,并能提高肝脏 GST活性 ,升高 GSH含量。说明原花青素对化学诱变剂引起的染色体断裂有一定保护作用 ,其作用机理可能与提高肝脏的解毒功能有关。The antimutagenic effect of procyanidins(PV 2000) on cyclophosphamide(CPA) inducing micronucleus of PCEs in mice bone marrow and the effect on glutathione S transferase (GST) activity,glutathione (GSH) level in the liver of mice were investigated. The result showed that the CPA induced micronucleus rate were inhibited from 18.06% to 48.69%. The GST activity and GSH level increased obviously. The experiments suggest that procyanidins have the effect of inhibiting mutagenesis and is probably related to improving the detoxifying function of the liver.

关 键 词：原花青素 中药 葡萄籽 抗诱发 微核实验 

分 类 号：R285.5[医药卫生—中药学]