痰热清注射液对早期急性放射性肺损伤大鼠肺组织水通道蛋白1表达的影响  

Effect of Tanreqing on expression of aquaporin-1 in rats with radiation-induced lung injury

作　　者：刘大伟[1] 钟文[1] 孙成英[1] 刘晓岚[2] 

机构地区：[1]中国医科大学附属第四医院肿瘤治疗中心放射治疗科,沈阳110032 [2]吉林大学附属第二医院肿瘤放疗科

出　　处：《肿瘤研究与临床》2012年第6期369-372,共4页Cancer Research and Clinic

摘　　要：目的观察痰热清注射液对急性放射性肺损伤大鼠水通道蛋白1（AQP1）表达的影响，探讨痰热清注射液对大鼠急性放射性肺损伤的作用机制。方法将20只SD大鼠随机分为地塞米松组（5mg／kg）、痰热清组（0．67ml／kg）、单纯照射组（0．9％NaC1溶液0．2m1）、正常对照组（0．9％NaC1溶液0．2m1），每组5只；肺照射剂量为17Gy，照射后1d开始腹腔注射，7d后处死大鼠。对肺组织行HE染色观察病理变化，行免疫组织化学及反转录-聚合酶链反应（RT．PCR）观察AQP1的表达。结果地塞米$,zLeR、痰热清组炎症改变较单纯放疔组有所减轻。免疫组织化学显示放疗后AQP1表达减低（P〈0．05），而地塞米松及痰热清治疗之后AQP1表达升高，与放疗后相比P〈0．05。RT—PCR显示正常对照组AQP1呈高表达（0．8006±0．05846），放疗后表达降低（0．4938±0．03477），地塞米松组、痰热清组照射后7dAQP1表达较单纯放疗组增加（0．6492±0．03477、0．6664±0．03251），差异有统计学意义（P〈0．05）。结论痰热清注射液对放射性肺损伤有一定的治疗作用，其作用机制可能与调节AQP1的表达有关。Objective To observe the expression of aquaporln-1（AQP1） in rats with acute lung injury induced by radiation, and discuss the protection of Tanreqing for rats with acute lung injury. Methods A total of 20 SD healthy rats were randomly assigned into 4 groups, including the comparison group, the radiation group（0.9% NaC1 0.2ml）, the dexamethasne group（Smg/kg） and the Tanreqing group（0.67ml/kg）. Strat intraperitoneal injection one day after radiotherapy,the rats were sacrificed after 7 days.The pathological changes of lung tissues were observed by HE stain.AQP1 expressions in lung tissues were detected by immunohistochemistry and RT-PCR test. Results Compared with the radiation group, there was an obvious amelioration in pathological injury of lung tissue in the dexamethasne and Tanreqing groups.AQP1 was increased significantly in comparison group （0.8006±0.05846）,but AQP1 was decreased in the radiation group （0.4938＋0.03477）（P〈0.05）, and AQP1 was increased after the Tanreqing and dexamethasne treatment（0.6492± 0.03477, 0.6664±0.03251） （P〈0.05）. Conclusion Tanreqing may have beneficial effects on radiation- induced lung injury by regulating AQP1 expression in the lungs of rats.

关 键 词：痰热清注射液 放射性肺损伤 水通道蛋白-1 

分 类 号：R273[医药卫生—中西医结合]