同种异体抗原和巨细胞病毒抗原诱导的树突状细胞基因表达差异  

Differential expression of genes in dendritic cell induced by human alloantigens and cytomegalovirus antigen

作　　者：邹秀平[1] 陈旺兵[1] 刘珊珊[1] 黄伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液内科,武汉430030

出　　处：《白血病．淋巴瘤》2012年第6期330-334,共5页Journal of Leukemia & Lymphoma

基　　金：基金项目：国家自然科学基金（81101572）

摘　　要：目的探讨人类同种异体抗原和巨细胞病毒抗原诱导的树突状细胞（DC）基因表达差异，寻找预防移植物排斥反应和巨细胞病毒（CMV）感染的分子靶点。方法采用基因芯片技术检测人类同种异体抗原和CMV抗原3A（CMV3A）诱导的DC基因表达差异。结果DC在同种异体抗原和CMV3A诱导下基因表达谱存在明显的差异，这些差异表达基因涉及许多信号通路：抗原处理和提呈通路、细胞色素p450代谢外源性的化学物质通路、Toll样受体信号通路、细胞运动和迁移通路、细胞因子／细胞因子受体作用通路；多个热休克蛋白（HSP）家族基因包括HSPA5、HSPA8、HSPA9B和HSP90AB1，在同种异体抗原的刺激下表达升高，而在CMV3A刺激下表达无明显变化。结论多个HSP家族基因在同种异体抗原诱导下的DC中表达上调，而在CMV3A诱导下无明显变化，这些基因可能在预防移植物排斥反应和CMV感染中有重要作用。Objective To investigate differential gene expression in dendritic cell（DC） in response to human alloantigen and cytomegalovirus protein, and search target genes which can prevent graft rejection and cytomegalovirus （CMV） infection. Methods Genome-wide microarray analysis was performed to test gene expression in DC in response to human alloantigen and cytomegalovirus protein 3A（CMV3A）. Results The results showed that the difference of gene expression of DC induced by CMV3A and alloantigen was significant. The genes with differential expression included antigen processing and presentation, toll-like receptor signaling pathway and cell movement/migration, cytokine-cytokine receptor interaction and metabolism of xenobiotics by cytochrome p450, and several heat shock protein（HSP） family members including HSPA5, HSPA8, HSPA9B and HSP90AB1 ,in DC induced by alloantigen were higher expression than that by CMV3A. Conclusion This study found several genes including heat shock protein family in DC induced by alloantigen were higher expression than that by CMV3A, these genes might play a valuable role in preventing graft rejection and CMV infection.

关 键 词：树突状细胞 同种异体抗原 巨细胞病毒抗原 急性移植物抗宿主病 寡核苷酸 序列分析 

分 类 号：R3[医药卫生—基础医学]