低分子肝素对COPD模型大鼠肺组织HIF-1α、HO-1及VEGF表达的影响  

EFFECTS OF LOW-MOLECULAR-WEIGHT HEAPRIN ON HYPOXIA INDUCIBLE FACTOR-1 ALPHA,HEME OXYGENASE-1 AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN LUNG TISSUE OF RAT MODEL WITH CHRONIC OBSTRUCTIVE PULMOANRY DISEASE

作　　者：郝敏[1] 韩伟忠[1] 程兆忠[1] 佟丽[1] 孙妮娜[1] 娇文娟[1] 

机构地区：[1]青岛大学医学院附属医院呼吸内科,山东青岛266003

出　　处：《齐鲁医学杂志》2012年第3期233-235,共3页Medical Journal of Qilu

摘　　要：目的研究低分子肝素(LMWH)对慢性阻塞性肺疾病(COPD)模型大鼠肺组织中低氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)、血红素加氧酶-1(HO-1)表达的影响。方法将60只SPF级Wistar大鼠随机分为正常对照组、COPD模型组及LMWH干预组。采用烟熏联合气管内注入脂多糖的方法建立COPD动物模型;LMWH组在建立COPD模型同时皮下注射低分子肝素干预。观察3组大鼠肺组织病理变化,并应用免疫组化的方法检测LMWH干预前后肺组织中HIF-1α、VEGF、HO-1的表达情况。结果 COPD组及LMWH组大鼠肺组织中HIF-1α、VEGF及HO-1的表达均较正常对照组明显升高,差异有显著性(F=58.97～88.12,q=6.70～18.65,P<0.01);与COPD模型组相比,LMWH干预组大鼠肺组织的病理改变较轻,HIF-1α、VEGF及HO-1表达下降,差异有显著性(q=8.64～11.19,P<0.01)。结论 HIF-1α、VEGF和HO-1可能均参与COPD发生发展,参与气道炎症及气道重塑;LMWH可以减轻气道炎症及气道重塑。Objective To study the influence of low-molecular-weight heparin （LMWH） on the expressions of hypoxia inducible factor-α（HIF-α）, heine oxygenase-1 （HO-1） and vascular endothelial growth factor （VEGF） in lung tissue of rats with chronic obstructive pulmonary disease （COPD）. Methods Sixty Wistar rats were randomized to control group,COPD mod- el group and LMWH-treated group. A COPD model in rats was created by intratracheal instillation of lipopolysaccharides and ex posed to cigarette smoking. Subcutaneous injection of LMWH was given to the rats in LMWH group immediately upon completion of the model. The pathology of Lung tissue of the rats in the three groups were observed, and the expressions of HIF-I~, HO-1 and VEGF were detected using immunohistochemical method. Results Compared with the control group, the expressions of HIF-a, VEGF and HO-1 were significantly elevated （F=58.97 88.12 ;q=6. 70--18. 65 ;P^0.01）. Compared with COPD model group, the pathological change of the lung tissue in LMWH treated group was milder, the expressions of HIF-α, VEGF and HO-1 signifi- cantly decreased （q=8. 64--11. 19,P〈0. 01L Conclusion HIF-1α,VEGF and HO-1 may involve in the development of COPD, airway inflammation and remodeling. LMWH can alleviate the inflammation and remodeling process of the airway.

关 键 词：低氧诱导因子-1Α 血管内皮生长因子类 血红素加氧酶-1 肺疾病 慢性阻塞性 肝素 低分子质量 

分 类 号：R563[医药卫生—呼吸系统]