基于无线刺激与遥测系统的心房颤动动物模型  

An animal model of atrial fibrillation by means of stimulation and telemetry system based on wireless

作　　者：陈雯雯[1] 陈颖敏[1] 徐瑾[1] 罗章源 张文赞 严国锋[3] 

机构地区：[1]上海交通大学医学院附属仁济医院心内科,上海200001 [2]杰升生物科技(上海)有限公司 [3]上海交通大学医学院实验动物科学部

出　　处：《中国心脏起搏与心电生理杂志》2012年第3期241-245,共5页Chinese Journal of Cardiac Pacing and Electrophysiology

基　　金：上海市科委资助项目(项目编号:09140901800)

摘　　要：目的探索一种可控、可靠、安全的心房颤动(简称房颤)动物模型的建立方法。方法自主设计并制作一种基于Microchip公司的PIC18(Peripheral Interface Controller,外围接口控制器)系列单片机和TI公司的RF(Radio Frequency,射频)无线收发芯片CC1101 CC1101(Chipcon公司)的植入式遥测刺激器,植入比格犬背部,记录电极留置腹侧皮下,刺激电极缝合于左心耳上,在犬清醒自由的条件下,通过计算机专用心电软件连续监测体表II导联心电信号,并发放间歇(刺激1 s,暂停2 s)高频(频率30 Hz)阈上(强度2 mA,脉宽5 ms)刺激,若间歇期内出现房颤,则人为干预中止刺激,若转为窦性心律,则继续刺激。结果植入式遥测刺激器在体外可稳定工作(包括采集模拟心电信号和发放刺激)30天,植入犬体内刺激3天后可诱导出房颤,持续时间最长达8 h。结论应用植入式遥测刺激器是建立房颤动物模型的可行性方法之一。Objective To explore a controllable, reliable and safe method to establish an animal model of atrial fibrilla- tion. Method We designed an implantable telemetry and stimulator based on Microchip＇s microcontroller of PIC 18 se- ries and TI＇s CC1101 RF wireless transceiver chip independently ,which we implanted into the back of the Beagle. The re- cording electrodes were left under the ventral skin while the stimulating electrodes were sutured on the surface of the left at- rial appendage. We monitored the surface Ⅱ lead electrocardiograph continuously through computer software and emitted intermittent （to stimulate 1 s and pause 2 s）, high frequency （30 Hz）, and up the threshold （intensity of 2 mA and pulse width of 5 ms） stimulations. If atrial fibrillation was observed, we suspended the stimulations, otherwise we continued the stimulations. Results In vitro, the implantable telemetry and stimulator could run stably for 30 days, including record- ing analogous electrocardiograph and emiting stimulations. Atrial fibrillation was successfully induced in the survival Beagle after 3 days stimulating. The longest duration of atrial fibrillation was 8 hours. Conclusion Implantable telemetry and stimulator can be feasible in the establishment of animal model of atrial fibrillation.

关 键 词：心血管病学 植入式 程控 遥测 心房颤动 模型 

分 类 号：R541.75[医药卫生—心血管疾病]