高糖、高脂诱导大鼠肝脏胰岛素抵抗评价  

Comparison of High Fat and/or High Sugar-induced Hepatic Insulin Resistance in Rats

作　　者：王俊贤[1] 李小军[1] 刘跃辉[2] 李玉桑[1] 杨艳羚[1] 唐和斌[1] 

机构地区：[1]中南民族大学药学院,武汉430074 [2]广东省佛山市高明区人民医院

出　　处：《中国药师》2012年第6期747-750,共4页China Pharmacist

基　　金：国家自然科学基金资助项目(编号:81101538);武汉市科技攻关计划项目(编号:201060623266);中南民族大学基本科研业务费专项资金项目(编号:CZQ11037);中南民族大学科学研究基金(编号:XTZ10001)

摘　　要：目的:比较高糖、高脂等不同饮食诱导大鼠肝脏胰岛素抵抗模型的差异。方法:SD大鼠分成4组,分别给予普通、高糖、高脂、高糖高脂饲料,12周后检测各组空腹血糖水平、葡萄糖耐量、空腹血清胰岛素水平(FI)、胰岛素抵抗指数(HOMA-IR)、肝糖原含量及肝脏葡萄糖转运体2(GLUT2)、葡萄糖转运体4(GLUT4)表达水平。结果:口服糖耐量试验中,高脂组、高糖高脂组血糖曲线下面积分别为(91.6±5.5)和(106.1±4.6)mmol.min.L-1,较对照组明显增大(P<0.05或0.001)。高脂组的FI与HOMA-IR分别为(20.2±0.9)μIU.L-1和(5.1±0.3),高糖高脂组则分别为(31.4±2.0)μIU.L-1和(8.2±0.7),均较对照组显著升高(P<0.05或0.001)。高糖高脂组的肝糖原含量为(1.3±0.0)mg.g-1,也较对照组显著增多(P<0.01)。高脂组和高糖高脂组的GLUT2表达分别为(75.8±4.0)%和(60.0±4.5)%,均比对照组明显降低(P<0.05或0.001);高糖、高脂、高糖高脂组的GLUT4表达分别为(60.6±5.2)%,(72.3±3.8)%,(57.1±2.9)%,同样较对照组显著降低(P<0.001)。结论:高糖结合高脂饮食更适合胰岛素抵抗模型的制备。Objective： To compare the differences between high fat and/or high sugar-induced insulin resistance model in rats. Method： Spragne-Dawley rats were divided into four groups and fed with common, high-sugar （HS） , high-fat （HF） or high-sugar high-fat（HSF） diet for 12 weeks. Fasting blood glucose levels, glucose tolerance, fasting serum insulin levels （FI） , homeostasis mod- el assessment of insulin resistance （ HOMA-IR）, hepatic glycogen contents and expression levels of GLUT2 and GLUT4 in livers were evaluated in each group. Result： In oral glucose tolerance test, the area under the curve of time-blood glucose of HF （ （ 91.6 ± 5.5 ） mmol·min·L-1 , P 〈0.05） and HSF （ （ 106.1 ±4.6） mmol·min·L-1 , P 〈0.001 ） groups were significantly larger than that of the control group. In comparison to FI and HOMA-IR in control group, both of the levels were significandy higher in HF （ （20.2 ±0.9） μIU·L-1, 5.1 ±0.3,P 〈 0.05 ） and HSF （ （31.4 ± 2.0） μIU·L-1, 8.2 ± 0.7, P 〈 0.001 ） groups. The hepatic glycogen contents of rats in HSF group were significantly lower （（ 1.3 ±0.0） mg ·g^-1, p 〈0.01 ） than that in control group. The expression levels of GLUT2 protein in rats＇ liver in HF （75.8% ±4.0% , P 〈 0.05 ） and HSF （60.0% ± 4.5% , P 〈 0.001 ） groups were sig- nificantly lower than that in control group. The expression levels of GLUT4 protein of rats in HS （60.6% ±5.2% , P 〈0.001 ） , HF （72.3% ±3.8% , P 〈0.001 ） and HSF （57.1% ±2.9% , P 〈0.001 ） groups were all significantly lower than that of control group. Conclusion： The results indicated that the high-sugar high- fat dlet could establish a more successful rat model of hepatic insulin resist,

关 键 词：高糖 高脂 肝脏胰岛素抵抗 葡萄糖转运体2 葡萄糖转运体4 

分 类 号：R965.1[医药卫生—药理学]