救必应中长梗冬青苷提取工艺正交试验优化  

Extraction Optimization of Pedunculoside in Ilex rotunda Thunb.by Orthogonal Test

作　　者：王海森[1] 李超生[2] 王晶[2] 李喆[3] 于蔷蔷[1] 华山[3] 谷雨[1] 

机构地区：[1]吉林农业大学,长春130118 [2]吉林省中医药科学院 [3]延边大学

出　　处：《中国药师》2012年第6期805-807,共3页China Pharmacist

基　　金：吉林省科技发展计划资助项目(编号:20090927)

摘　　要：目的:研究长梗冬青苷最佳提取工艺条件。方法:采用正交试验设计研究不同浓度的乙醇、乙醇体积、提取时间和提取次数对长梗冬青苷提取率的影响;采用高效液相色谱法测定提取物中长梗冬青苷含量。结果:救必应中长梗冬青苷最佳提取条件为加入6倍量70%乙醇,回流提取3次,每次1 h。结论:本法提取效率高,适合大量提取,可用于工业化生产。Objective： To optimize the extraction technology of pedunculoside in Ilex rotunda Thunb. Method： Alcohol with differ- ent concentrations as the solvent, the amount of the solvent, the extraction time and the extraction frequency were optimized by orthogo- nal test with the extraction rate of pedunculoside as the index. Pedunculoside content was determined by HPLC. Result： The optimum extraction conditions were as follows：6 times of 70% alcohol as the solvent,reflux extraction 3 times with lh each. Conclusion： The procedure is efficient with lower energy and shortened time. It is suitable for the industrial production.

关 键 词：正交试验 救必应 长梗冬青苷 提取工艺 

分 类 号：R284.2[医药卫生—中药学]