高效液相色潽法检测复方二氧丙嗪茶碱片中茶碱含量  

Determination of Theophylline in Compound Dioxopromethazine Hydrochloride Tablets by RP-HPLC

作　　者：孙洪刚[1] 王颖[1] 李东伟[1] 陈笑强[1] 张椿影[1] 崔雪丹[1] 贾首时 

机构地区：[1]齐齐哈尔市第一医院,黑龙江齐齐哈尔161005 [2]齐齐哈尔市药品检验所

出　　处：《中国药师》2012年第6期824-825,共2页China Pharmacist

摘　　要：目的:建立高效液相色谱法检测复方二氧丙嗪茶碱片中茶碱含量。方法:色谱柱为Kromasil-C18(250 mm×4.6 mm,5μm);流动相为水-甲醇(65∶35);流速为1.0 ml·min-1;检测波长:275 nm。结果:茶碱在0.107～5.335μg·ml-1范围内,与峰面积值呈良好的线性关系,r=0.999 7;平均加样回收率为100.1%,RSD=0.36%(n=9)。结论:方法简便、准确、重复性好,可用于该制剂的含量测定。Objective： To establish a method for the determination of theophylline in compound dioxopromethazine hydrochloride tablets by HPLC. Method： The column was Kromasil-C18 （250 mm × 4.6 mm ,5μm）. The mobile phase was water-methanol （65： 35）. The flow rate was 1.0 ml · min^-1 with the detection wavelength of 275 rim. Result： The linear range of theophylline was 0. 107- 5. 335 μg · ml^-1, r = 0. 999 7. The average recovery was 100.1% with RSD of 0.36% （n = 9）. Conclusion： The method is simple, accurate and repeatable.

关 键 词：茶碱 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]