探讨流式细胞术检测HLA-B27在强直性脊柱炎中的临床应用价值  

Evaluate the clinical value of HLA-B27 detecting by flow cytometry for ankylosing spondylitis

作　　者：裴兵[1] 

机构地区：[1]江苏省泗洪县人民医院检验科,泗洪县223900

出　　处：《实用检验医师杂志》2012年第1期31-34,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的以序列特异性引物一聚合酶链反应（sequence-specificprimerpolymerasechainreac-tion．PCR-SSP）作为金标准，探讨贝克曼一库尔特EPICSXLHLA-B27在强直性脊柱炎中的临床诊断价值。方法上海交通大学附属瑞金医院进行HLA-B27流式细胞术检测的患者187例，分别进行流式细胞术HLA-B27抗原检测和PCR-SSP基因型检测，并对检测结果进行分析。结果在187份AS患者标本中，DNA抽提合格标本157份。PCR-SSP结果显示58份阳性，99份阴性，流式细胞术结果显示阳性标本60份．阴性标本97份。以PCR-SSP为金标准，流式细胞术检测HIA-B27的灵敏度为100．O％，特异性为96．7％，准确度为98．7％。结论流式细胞仪检测HLA-B27对AS有较高的灵敏度与特异性，适合于临床大规模样本量的筛查。Objective To evaluate the Beckman-Coulter EPICS XL HLA-B27 detection system of flow cytometry for detecting ankylosing spodylitis （AS） by sequence-specific primer polymerase chain reaction （PCR-SSP） as a gold standard. Methods 187 cases clinical specimens detecting HLA-B27 by flow cytome- try were collected from AS patients in Shanghai Jiaotong University, Ruijing hospital. HLA-B27 antigen detec- tion and PCR-SSP genotyping detection were done and the results were analyzed. Results In the 187 speci- mens, DNA extraction qualified samples were 157 cases. PCR-SSP showed that 58 cases were positive, 99 cases were negative, and flow cytometry showed positive samples were 60 cases, negative specimens were 97 cases. By PCR-SSP as the gold standard, the sensitivity of flow eytometry detecting HLA-B27 were 100.0%, specificity 96.7%, accuracy 98.7%. Conclusion HLA-B27 detection by flow eytometry has high sensitivity and specificity which suitable for large sample screening in chinical.

关 键 词：强直性脊柱炎 PCR-SSP 流式细胞术 HLA-B27 

分 类 号：R593.23[医药卫生—内科学]