单纯孕激素口服避孕药对大鼠催乳素水平及垂体催乳素细胞的影响  

THE EFFECTS OF PROGESTIN - ONLY CONTRACEPTION ON THE PRL LEVEL AND THE MORPHOLOGICAL CHANGE OF THE PITUITARY PRL CELLS IN RATS

作　　者：黎海莉[1] 潘莹[2] 申薇[1] 杜鹃[2] 康林[2] 崔慧先[2] 

机构地区：[1]河北医科大学第四医院妇产科,河北石家庄050011 [2]河北医科大学基础医学院人体解剖学教研室,河北石家庄050017

出　　处：《河北医科大学学报》2012年第6期621-624,F0002,共5页Journal of Hebei Medical University

基　　金：河北省科技厅博士基金课题(04547002D-3)

摘　　要：目的探讨单纯孕激素口服避孕药左旋18-甲基炔诺酮(levonorgestrel,LNG)对大鼠垂体催乳素(prolactin,PRL)细胞形态以及血清浓度的影响。方法正常雌性SD大鼠分为长期给药组和停药组,应用免疫组织化学和透射电镜方法,观察大鼠垂体PRL细胞形态变化并检测PRL的血清浓度。结果长期给药组PRL免疫反应阳性细胞数目增多(P<0.01)、面积增大(P<0.05),血清PRL升高(P<0.01),电镜下可见PRL细胞高尔基复合体发达、粗面内质网丰富,合成与分泌旺盛。停药后各项观察指标接近正常。结论应用LNG可引起大鼠垂体PRL细胞的形态、PRL的合成与分泌及血清PRL浓度的改变,但这一过程是可恢复的。LNG使血清PRL浓度升高,对排卵有一定抑制作用。Objective To investigate the morphological alternations of prolactin （PRL）cells and the serum concentrations of PRL in levonorgestrel （LNG） - treated rats. Methods The normal female SD rats were divided into long - term treated group and drug withdrawal group. The pituitary PRL cells were identified by immunohistochemistry and transmission electron microscope （TEM） method. The serum concentrations of PRL were measured by IMMULITE automated analyzer. Results In long - term treated group,the number and mean areas of PRL cells increased （P 〈 0.01, P 〈 0.05） and the shape became irregular. Golgi complex and rough endoplasmic reticulum became very developed and the secretory granules with various size and shape decreased. The serum concentrations of PRL were significantly higher in treated group than those of control group（P 〈 0.01 ）. After drug - withdrawal, these observation indexes recovered to normal gradually. Conclusion LNG causes changes of morphology of PRL cells and concentrations of PRL,but these changes can return to normal after ceasing treatment. The antiovulation of LNG is various and complex and involve in the increase of the serum concentrations of PRL.

关 键 词：避孕药 口服 激素 垂体 催乳素 大鼠 

分 类 号：R979.21[医药卫生—药品]