Effects of Electroacupuncture plus Dopamine D1 Receptor Antagonist on Somatosensory Evoked Potentials and Behavioral Changes in Rats with Cerebral Ischemia-reperfusion  

电针联合多巴胺D1受体拮抗剂对脑缺血-再灌注后大鼠体感诱发电位及行为学的影响(英文)

作　　者：刘成勇 徐鸣曙 葛林宝 

机构地区：[1]Shanghai University of Traditional Chinese Medicine [2]Shanghai Research Institute of Acupuncture and Meridian [3]Shanghai Research Institute of Qigong [4]Shanghai Research Center of Acupuncture and Meridian

出　　处：《Journal of Acupuncture and Tuina Science》2012年第3期133-137,共5页针灸推拿医学（英文版）

基　　金：supported by National Basic Research Program of China(973 Program,2009CB522900);National Natural Science Foundation of China(81001547);Shanghai Leading Academic Discipline Project(S30304)

摘　　要：Objective： To explore the effects of electroacupuncture （EA） plus dopamine D 1 receptor （D 1 R） antagonist on somatosensory evoked potentials （SEP） and behavioral changes in rats with cerebral ischemia- reperfusion. Methods： Forty rats were randomly divided into a normal group, a model group, an EA group, a D1R antagonist group and an EA＋D1R antagonist group, with 8 rats in each group. Ischemia-reperfusion model was established in the other 4 groups except the normal group, and they were treated by different ways after modeling successfully. SEP, neural function defect score （NFDS） and beam walking tests were performed before and after modeling, before sacrifice respectively. Results： SEP of the EA group, D1R antagonist group and EA＋D1R antagonist group showed significant differences when compared with that of the model group （P〈0.05） after treatment. Before treatment, behavioral tests of the model group showed significant decreases compared with those of the normal group （P〈0.05）. After treatment, behavioral tests in the EA group, the D 1R antagonist group and the EA＋D 1R antagonist group improved obviously and showed statistical differences compared with those in the model group （P〈0.05）. Conclusion： EA and D1R antagonist treatments could both promote the neural functional recovery after ischemia-reperfusion and play a brain-protective role, but there was no synergistic effect of EA and D 1R antagonist in combination.目的:研究电针联合多巴胺D1受体(D1 Receptor,D1R)拮抗剂对脑缺血再灌注后大鼠体感诱发电位(Somatosensory Evoked Potential,SEP)及行为学的影响。方法:将40只雄性SD大鼠随机分为正常组、模型组、电针组、D1R拮抗剂组、电针+D1R拮抗剂组,每组8只。除正常组外,其余4组制作大鼠脑缺血-再灌注模型,模型成功后予相应处理。分别于处理前、造模即刻、实验结束时检查大鼠SEP,并进行神经功能缺损评分(Neural Function Defect Score, NFDS)和平衡木试验。结果:治疗后电针组、D1R拮抗剂组及电针+D1R 拮抗剂组SEP均有明显恢复,与模型组差异均有统计学意义(P<0.05)。模型组行为学评分有明显降低,与正常组有统计学差异(P<0.05)。治疗后,电针组、D1R拮抗剂组及电针+D1R拮抗剂组有明显改善,与模型组差异均有统计学意义(P<0.05)。结论:电针与D1R拮抗剂均能促进脑缺血损伤后神经功能恢复,具有脑保护作用,联合使用没有发生协同叠加作用。

关 键 词：Acupuncture Therapy ELECTROACUPUNCTURE Brain Ischemia Reperfusion Injury DopamineAntagonists Evoked Potentials  Somatosensory RATS 

分 类 号：R203[医药卫生—中医学]