深海芽孢杆菌E401B03次生代谢产物分离和结构鉴定  

Isolation and identification of secondary metabolites produced by a deep-sea Bacillus sp. E401B03

作　　者：郭书举[1] 史大永[1] 李富超[1] 朱校斌[1] 缪宇平[2] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国水产科学院东海水产研究所,上海200090

出　　处：《海洋科学》2012年第6期28-30,共3页Marine Sciences

基　　金：农业部海洋与河口渔业资源及生态重点开放实验室开放课题(开-09-03);江苏省自然科学基金项目(BK2011395)

摘　　要：利用硅胶柱、凝胶柱及高效液相(HPLC)等色谱方法,对采自中国南海的深海芽孢杆菌E401B03发酵液的乙酸乙酯相浸膏进行了分离,通过1H-NMR、13C-NMR、EIMS等方法鉴定了7个化合物:N-苯乙基乙酰胺、3-(羟基乙酰基)-吲哚、3-(2-羟基乙基)-吲哚、环(缬-缬)二肽、尿嘧啶、胸腺嘧啶、对羟基苯甲醛。Seven compounds, were isolated from the ethyl acetate extract of Bacillus sp. E401B03. Compounds 1-7 were obtained by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were identified by spectroscopic methods including 1H-NMR,13C-NMR, and EIMS.

关 键 词：深海 芽孢杆菌 次生代谢产物 

分 类 号：R931.77[医药卫生—生药学]