抗3种β-激动剂双特异性单克隆抗体的建立及鉴定  

Establishment and identification of bispecific monoclonal antibody against three kinds of β-agonists

作　　者：余厚美 崔廷婷 冯才伟 万宇平 

机构地区：[1]北京勤邦生物技术有限公司,北京102206

出　　处：《化学试剂》2012年第7期607-611,共5页Chemical Reagents

摘　　要：制备抗克仑特罗、沙丁胺醇和莱克多巴胺双特异性抗体并初步鉴定。将分泌抗克仑特罗、沙丁胺醇抗体的细胞株和分泌抗莱克多巴胺抗体的细胞株分别驯化,使之成为HAT敏感株。将两者融合,筛选分泌双特异性单克隆抗体的杂交-杂交瘤株,然后对其进行初步鉴定。共获得5株杂交-杂交瘤细胞株,将其分泌的双特异性单克隆抗体(BsAb)经过酶联免疫吸附法(ELISA)初步检测,IC50可达1 ng/mL,腹水效价为105以上,用ELISA法证明BsAb只与克仑特罗、沙丁胺醇和莱克多巴胺有特异性反应。该方法制备简单、风险低、灵敏度高、周期短,具有较好的应用前景。To prepare and identify bispecific monoclonal antibody（BsAb） against three kinds of β-agonists（clenbuterol,salbutamol,ractopamine）.Hybridoma cells secreting anti-clenbuterol and salbutamol mAb and hybridoma cells secreting anti-ractopamine mAb were naturalized respectively,making them sensitive to HAT.Then the two hybridoma cells sensitive to HAT were fused and hybrid-hybridoma cells secreting the BsAb were screened and identified.5 hybrid-hybridoma cell lines were obtained.The sensitivities and specificities of BsAb were analyzed by ELISA,showed that the IC50 was 1 ng/mL,the titer of ascites were over 105.They can only react with clenbuterol,salbutamol and ractopamine.This method had higher sensitivity and simplicity,lower risk,and can be used widely.

关 键 词：双特异性单克隆抗体(BsAb) 克仑特罗 沙丁胺醇 莱克多巴胺 

分 类 号：R392.11[医药卫生—免疫学]