龙胆药材及龙胆总苷提取物的HPLC指纹图谱研究  

RP-HPLC fingerprint for qualitative assessment of Radix gentianae and its extracts

作　　者：秦晶[1] 陈国锋[1] 韩丽妹[1] 马琳怡[1] 王建新[1] 

机构地区：[1]复旦大学药学院药剂学教研室,上海201203

出　　处：《中国新药杂志》2012年第13期1466-1469,1478,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09310-006);科技部"科技人员服务企业行动"项目(2009GJC00020)

摘　　要：目的:建立龙胆药材及龙胆总苷提取物的指纹图谱,为有效控制药材及提取物质量提供可靠方法。方法:采用磷酸-乙腈系统梯度洗脱,建立龙胆药材及提取物的指纹图谱。结果:建立了21批不同产地龙胆药材指纹图谱的共有模式,确立19个共有峰,通过比对证明提取物与药材的共有峰具有同源性。10批龙胆总苷提取物指纹图谱相似度>0.98。结论:龙胆药材与总苷提取物指纹图谱特征性及专属性强,具有较好的同源性,可用于二者的质量控制。Objective： To establish a method of chromatography fingerprint for detecting Radix gentianae and the total glucosides extracted from Radix gentianae. Methods： RP-HPLC with gradient elution was employed to establish chemical fingerprint. The investigated constituents were authenticated by comparing their retention times with those of reference compounds. Results： Nine common peaks were found in HPLC-fingerprint of Radix genti- anae and the extracts. The fingerprints of 10 batches of extract were highly similar with similarity degree higher than 0.98. Conclusion： The proposed approach with good specificity can be utilized as a comprehensive quality control method for Radix gentianae and its extracts

关 键 词：龙胆 龙胆总苷 指纹图谱 分离纯化 质量控制 

分 类 号：R284.2[医药卫生—中药学]