蓬子菜总黄酮对过氧化氢损伤的人脐静脉内皮细胞的保护作用  

Protective effect of flavones from Galium verum L on human umbilical vein endothelial cells injured by hydrogen dioxide

作　　者：史灵恩[1] 凌秋[2] 杨晓蕾[1] 李海霞[1] 马玲[1] 

机构地区：[1]哈尔滨医科大学流行病教研室,哈尔滨150081 [2]哈尔滨医科大学微生物教研室,哈尔滨150081

出　　处：《中国新药杂志》2012年第13期1523-1527,共5页Chinese Journal of New Drugs

基　　金：黑龙江省科技攻关项目(GC03C402-1)

摘　　要：目的:探讨蓬子菜总黄酮对人脐静脉内皮细胞(human umbilical vein endothelial cells,HU-VECs)的保护及损伤修复的作用。方法:采用台盼蓝染色法,观察不同剂量蓬子菜总黄酮(12.5～400μg.mL-1)对HUVECs生长的影响;建立过氧化氢(H2O2)诱导HUVECs氧化损伤模型,采用MTT比色法检测细胞增殖,酶联免疫分析方法检测内皮素-1(endothelin,ET-1)和降钙素基因相关肽(calcitonin generelated peptide,CGRP)的分泌水平。AO/EB双染色法观察HUVECs的凋亡。结果:与模型组比较,蓬子菜总黄酮能减轻H2O2对HUVECs增殖抑制作用,促进CGRP的分泌,降低ET-1的分泌水平,抑制HUVECs的凋亡。结论:蓬子菜总黄酮对氧化损伤的HUVECs具有保护及修复作用,其机制与调节ET-1和CGRP的分泌有关。Objective：To study the protecting and anti-apoptosis effects of flavones from Galium verum L （FGVL） on human umbilical vein endothelial cells （HUVECs） injured by hydrogen dioxide （H202）. Methods： The effects of different dosages of FGVL （12.5 -400μg-mL-1） on cell growth were evaluated by trypan blue stai- ning. The HUVECs injury model was induced by H202. The proliferation of HUVECs was measured by MTT assay, and the secretion levels of endothelin-1 （ET-1） and calcitonin gene related peptide （CGRP） were measured by ELISA. AO/EB double staining was used to detect the apoptosis. Results： Compared with the injury model, FGVL can reduce the proliferation inhibition of HUVECs. The production of ET-1 was decreased and the level of CGRP was obviously increased. The anti-apoptosis effect of FGVL was also observed. Conclusions： FGVL can protect HUVECs from oxidative damage, and the mechanism is related to the regulation of ET-1 and CGRP secretion.

关 键 词：蓬子菜总黄酮 人脐静脉内皮细胞 过氧化氢 内皮素-1 降钙素基因相关肽 凋亡 

分 类 号：R961[医药卫生—药理学]