酸性品红标记分光光度法测定微量人血清白蛋白  

Determination of Micro Human Serum Albumin by Acid Fuchsine Spectrophotometry

作　　者：赵丹华[1] 

机构地区：[1]广东第二师范学院化学系

出　　处：《光谱实验室》2012年第4期2273-2276,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：广州珠江科技新星专项;广东第二师范学院博士专项研究基金(10ARF12)

摘　　要：基于pH 1.86的B-R缓冲溶液条件下,酸性品红与人血清白蛋白(HSA)的结合使酸性品红在545nm处吸光度下降的原理,其吸光度的下降值(△A)与HSA在0—28.0μg/mL(r=0.9959)呈良好的线性关系。该方法用于合成试样测定,结果令人满意。同时对酸性品红与人血清白蛋白结合反应的机理进行了初探。The principle of decreasing absorbance of acid fuchsine in 545nm because of combining acid fuchsine with human serum albumin（HSA） in the Britton-Robinson buffer solution（pH=l.86）, and there was the good linear relationship between the drop-out value（△A） of absorbance and HSA concentration in the range of 0-28.0μg/mL（r = 0.9959）.The method can be applied to determination of the synthetized samples with the satisfactory results.The principle of reaction between acid fuchsine and HSA was firstly investigated.

关 键 词：酸性品红 人血清白蛋白 分光光度法 

分 类 号：O657.32[理学—分析化学]