分光光度法测定利福平磺丁基醚-β-环糊精包合物  

Determination of Rifampicin Sulfonylurea Butyl Ether-β-Cyclodextrin Inclusion Complex by Spectrophotometry

作　　者：尹华峰[1] 杨林 刘碧林[1] 刘国栋[3] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆市渝中区医学院路1号400016 [2]重庆市食品药品检验所,重庆市北部新区春兰二路1号401121 [3]第三军医大学大坪医院野战外科研究所第8研究室,重庆市渝中区长江支路10号400042

出　　处：《光谱实验室》2012年第4期2372-2375,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：重庆市教育委员会资助项目(首批高等学校优秀人才资助)

摘　　要：建立测定利福平磺丁基醚-β环糊精包合物中利福平含量的方法。采用分光光度法测定含量,测定波长为474nm。利福平在474nm处有最大吸收,在9.786—39.14μg/mL,范围内与吸光度呈良好的线性关系,回归方程为:A=0.01 72C+0.0025(r=0.9998);平均回收率为99.87%(n=9),RSD为0.24%;日内RSD分别为0.81%、0.43%、0.57%(n=5);日间RSD分别为1.06%、1.21%、1.53%(n=3);测得3批样品的含量分别为25.33%、25.98%、25.76%。本法准确、简便、迅速,适用于利福平磺丁基-β-环糊精包合物中利福平含量的测定。The method for the determination of the content of rifampicin sulfonylurea butyl ether-β-cyclodextrin inclusion complex was established.The content was determined by spectrophotometry,and the detection wavelength was 474nm.The rifampicin had the maximum absorption at 474nm,that had a good linear relationship in the range of 9.786-39.14μg/mL with absorption.The regression equation was A = 0.0172C ＋ 0.0025（r=0.9998）.The average recovery was 99.87%,RSD was 0.24%（n=9）.The within day RSDs were 0.81%,0.43%and 0.57%（n= 5）,and between-day RSDs were 1.06%,1.21%and 1.53%（n=3）.The contents of 3 batches of samples were respectively 25.33%,25.98%,25.76%.The method is simple,rapid and accurate, which can be used for determination of the content of rifampicin sulfonylurea butyl ether-β-cyclodextrin inclusion complex.

关 键 词：利福平 利福平磺丁基-β-环糊精包合物 分光光度法 

分 类 号：O657.32[理学—分析化学]