HPLC测定甲磺酸托烷司琼的有关物质  

Relevant Substance of Methanesulfonic Acid Tropisetron by HPLC

作　　者：石云龙[1] 孟祥军[2] 王凤秋 

机构地区：[1]沈阳医学院沈洲医院,沈阳市和平区北九马路20号110035 [2]沈阳医学院化学教研室 [3]辽宁卫生职业技术学院药学系,沈阳市苏家屯区乔松路2号110101

出　　处：《光谱实验室》2012年第4期2433-2437,共5页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立HPLC测定甲磺酸托烷司琼的有关物质。采用C18色谱柱,以甲醇-水-乙腈-三乙胺(565:40:35:0.3,V/V/V/V)为流动相A,以甲醇-水-乙腈-三乙胺(800:100;100:0.24,V/V/V/V)为流动相B,梯度洗脱检查有关物质;检测波长为284nm。甲磺酸托烷司琼检出限为0.24ng,主成分与其有关物质能完全分离。方法简便、准确、专属性好,可用于甲磺酸托烷司琼有关物质的测定。The method for determination of methanesulfonic acid tropistron by HPLC was established.The compound was separated by column C18 with methanol-water-acetonitriletriethylamine （565：40：35：0.3,V/V/V/V） as mobile phase A and methanol-water-acetonitriletriethylamine （800：100：100：0.3,V/V/V/V） as mobile phase B for gradient elute,and the detection wavelength was 284nm.The detection limit of methanesulfonic acid tropistron was 0.24ng, and the principal component can separated from the other relevant compounds.The method is simple, accurate with good specificity,and can be applied to determining the relevant compounds of methanesulfonic acid tropistron.

关 键 词：甲磺酸托烷司琼 有关物质 高效液相色谱法 

分 类 号：O657.7[理学—分析化学]