两种硫酸长春新碱脂质体包封率测定方法的比较  

Comparison of Two Methods for Determination of Entrapment Efficiency of Vincristine Sulfate Liposome

作　　者：李文静[1,2] 杨志强[1,3] 王杏林[1,3] 

机构地区：[1]释药技术与药代动力学国家重点实验室,天津300193 [2]天津中医药大学,天津300193 [3]天津药物研究院,天津300193

出　　处：《中国实验方剂学杂志》2012年第14期71-75,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基础研究发展计划项目(2010CB735602)

摘　　要：目的:建立两种测定硫酸长春新碱脂质体包封率的方法,并对测定结果进行比较。方法:分别以葡聚糖凝胶Sephadex G-50柱和阳离子交换树脂柱分离硫酸长春新碱脂质体和游离药物,采用HPLC法测定药物含量,计算包封率,并用SPSS软件进行t检验。结果:两种方法测定不同药脂比的硫酸长春新碱脂质体包封率,测定结果无显著性差异。结论:葡聚糖凝胶柱法的建立需详细考察凝胶的种类对分离度的影响;阳离子交换树脂法分离度良好,分离速度快,为优选的方法。Objective：Establish the two methods for the determination of entrapment efficiency of liposomes.Method： Sephadex G-50 and cation exchange resin were used to separate free vincristine sulfate from liposomes.The concentration of vincristine sulfate was determined by HPLC.The result was analyzed with SPSS.Result： The entrapment efficiency of the different drug loading liposome determined by the two methods was consistent.Conclusion： Both methods can be used to determine the entrapment efficiency of vincristine sulfate liposomes.

关 键 词：PH梯度法 硫酸长春新碱脂质体 葡聚糖凝胶柱法 阳离子交换树脂法 包封率 

分 类 号：R284.1[医药卫生—中药学]