HPLC定量特征图谱在补阳还五汤质量评价中的应用  

Application of Quantitative Characteristic Spectrum to Quality Evaluation of Buyanghuanwutang

作　　者：周琴妹[1] 张源[1] 陈武[1] 

机构地区：[1]江苏省中医院,南京210029

出　　处：《中国实验方剂学杂志》2012年第14期75-78,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项项目(201007010)

摘　　要：目的:采用定量特征图谱对补阳还五汤进行质量评价,以此来定量控制其煎煮液的质量。方法:采用HPLC法建立补阳还五汤特征图谱,并进行方法学考察,以芍药苷为参照峰,计算各共有峰的相对保留时间和相对峰面积的RSD,考察精密度、稳定性、重复性;建立10批补阳还五汤的特征图谱,并比较特征图谱的技术参数,通过计算其相关系数、夹角余弦和程度相似度,并结合数量相似度,综合评定了其质量。结果:精密度、稳定性、重复性考察中共有峰相对保留时间和相对峰面积的RSD均<3.0%;样品的相关系数和夹角余弦计算结果都在0.95以上,无法反映样品间的差异;而程度相似度的结果差异较大,能反映样品间的差异。用程度相似度结合数量相似度的方法比通过相关系数、夹角余弦等计算相似度的方法可更好的反映出不同批次样品间的差异,达到控制产品质量的目的。结论:该方法准确、可行,对中药质量评价是一种合理、有效的技术手段。Objective：To evaluate the quantitative characteristic spectrum to control the quality of buyanghwanwutang the product by extent.Method： The Characteristic spectrum of buyanghuanwutang was established.In methodological study,peoniflorin as the reference peak to calculate the RSD of the relative retention time and relative peak area of each common peaks,precision,stability and reproducibility were investigated as well.The characteristic spectrums of 10 batches of buyanghuanwutang were established.The quality of buyanghuanwutang was evaluated by the correlation,cosine of the angle and extent similarity of ten batches,combined with the numericalsimilarity.Result： RSD of the relative retention time and relative peak area of common peaks of precision,accuracy,repeatability was less than 3%.The calculation results of Samples＇ correlation coefficient and angle cosine are above 0.95,it cannot reflect the differences among samples,but there is a great difference in extent similarity,so it can reflect the differences among samples.The combination of the extent similarity and numerical similarity was better than the correlation,cosine of the angle.The combination reflected the differences of different batches,and was used to control the quality of products.Conclusion： This method is accurate and reliable,for quality evaluation of Traditional Chinese Medicine.

关 键 词：补阳还五汤 特征图谱 程度相似度 数量相似度 

分 类 号：R284.1[医药卫生—中药学]